Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Saccharomyces cerevisiae Galactose/lactose metabolism regulatory protein GAL80 (GAL80)

Recombinant Saccharomyces cerevisiae Galactose/lactose metabolism regulatory protein GAL80 (GAL80)

  • 货号：
    CSB-YP356247SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP356247SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP356247SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP356247SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP356247SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GAL80
  • Uniprot No.：
  • 别名：
    GAL80; YML051W; YM9827.01; YM9958.12Galactose/lactose metabolism regulatory protein GAL80
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-435
  • 氨基酸序列
    MDYNKRSSVS TVPNAAPIRV GFVGLNAAKG WAIKTHYPAI LQLSSQFQIT ALYSPKIETS IATIQRLKLS NATAFPTLES FASSSTIDMI VIAIQVASHY EVVMPLLEFS KNNPNLKYLF VEWALACSLD QAESIYKAAA ERGVQTIISL QGRKSPYILR AKELISQGYI GDINSIEIAG NGGWYGYERP VKSPKYIYEI GNGVDLVTTT FGHTIDILQY MTSSYFSRIN AMVFNNIPEQ ELIDERGNRL GQRVPKTVPD HLLFQGTLLN GNVPVSCSFK GGKPTKKFTK NLVIDIHGTK GDLKLEGDAG FAEISNLVLY YSGTRANDFP LANGQQAPLD PGYDAGKEIM EVYHLRNYNA IVGNIHRLYQ SISDFHFNTK KIPELPSQFV MQGFDFEGFP TLMDALILHR LIESVYKSNM MGSTLNVSNI SHYSL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar.
  • 基因功能参考文献：
    1. Here, the s demonstrate that altering the epistatic interaction between Gal80p and Gal4p also abolishes the bistability of the GAL/MEL genetic switch, and the switch response to galactose becomes graded instead of binary. PMID: 27573383
    2. GAL switch becomes independent of GAL3 by altering the interaction between Gal4p and Gal80p. PMID: 25135592
    3. to understand mechanism of GAL regulatory system, structure of Gal80p with activation domain of Gal4p was determined; structure reveals existence of a dinucleotide mediating interaction between the two; findings suggest NADP plays a key role PMID: 18292341
    4. Both MgATP and galactose are required to promote the formation of a 1:1 complex between Gal80p and Gal3p. PMID: 11964151
  • 数据库链接：

    KEGG: sce:YML051W

    STRING: 4932.YML051W