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  4. Recombinant Saccharomyces cerevisiae General control protein GCN4 (GCN4)

Recombinant Saccharomyces cerevisiae General control protein GCN4 (GCN4)

  • 中文名称：
    酿酒酵母GCN4重组蛋白
  • 货号：
    CSB-YP365837SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母GCN4重组蛋白
  • 货号：
    CSB-EP365837SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母GCN4重组蛋白
  • 货号：
    CSB-EP365837SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母GCN4重组蛋白
  • 货号：
    CSB-BP365837SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母GCN4重组蛋白
  • 货号：
    CSB-MP365837SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GCN4
  • Uniprot No.：
  • 别名：
    GCN4; AAS3; ARG9; YEL009C; General control protein GCN4; Amino acid biosynthesis regulatory protein
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-281
  • 氨基酸序列
    MSEYQPSLFA LNPMGFSPLD GSKSTNENVS ASTSTAKPMV GQLIFDKFIK TEEDPIIKQD TPSNLDFDFA LPQTATAPDA KTVLPIPELD DAVVESFFSS STDSTPMFEY ENLEDNSKEW TSLFDNDIPV TTDDVSLADK AIESTEEVSL VPSNLEVSTT SFLPTPVLED AKLTQTRKVK KPNSVVKKSH HVGKDDESRL DHLGVVAYNR KQRSIPLSPI VPESSDPAAL KRARNTEAAR RSRARKLQRM KQLEDKVEEL LSKNYHLENE VARLKKLVGE R
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Master transcriptional regulator that mediates the response to amino acid starvation. Binds variations of the DNA sequence 5'-ATGA[CG]TCAT-3' in canonical nucleosome-depleted 5'-positioned promoters, and also within coding sequences and 3' non-coding regions. During nutrient starvation (low or poor amino acid, carbon or purine sources), it activates genes required for amino acid biosynthesis and transport, autophagy, cofactor biosynthesis and transport, mitochondrial transport, and additional downstream transcription factors. Activates transcription by recruiting multiple coactivators, including the mediator complex, the SAGA complex, and the SWI/SNF complex, to enable assembly of the pre-initiation complex at core promoters.
  • 基因功能参考文献：
    1. The down-regulation of the GCN4 expression (Gcn(-) phenotype) in the eIF5(G31R) mutant was not because of leaky scanning defects; rather was due to the utilization of upUUG initiation codons at the 5' regulatory region present between uORF1 and the main GCN4 ORF. PMID: 28385532
    2. GCN4 is required for nitrogen catabolite repression-sensitive nuclear Gln3 localization. PMID: 28007891
    3. Gcn4 sumoylation in limiting its association with chromatin. PMID: 27770033
    4. Presented are molecular dynamics simulations reflecting a secondary structure of phopspho-GCN4 at different pH. PMID: 28230348
    5. Reinitiation on GCN4 mRNA after translation of its four short uORFs is regulated by the sequence immediately following their respective stop codons. PMID: 26822200
    6. Upstream-open reading frame 2 (uORF2) from the GCN4 mRNA leader resembles the well-established reinitiation (REI)-permissive uORF1 in its high REI-promoting activity. PMID: 24623812
    7. our results suggest that the folding behavior of the well-studied GCN4 leucine-zipper domain is more complex than was previously appreciated. PMID: 23116373
    8. Elucidated RNA binding interface of the GCN4 leucine zipper motif from yeast. Site-directed mutagenesis of the LZ-GCN4 RNA binding interface showed that substrate binding is facilitated by lysine and arginine side chains. PMID: 22374868
    9. the combination of Gln3 and Gcn4 results in the constitution of a hybrid modulator which elicits a novel transcriptional response, not evoked when these modulators act in a non-combinatorial fashion. PMID: 21184740
    10. Gcn4(L267S) variant carries an amino acid substitution in the third of four conserved leucines of the zipper dimerization domain. This abolished FLO11 expression and resulted in reduced transcriptional activity for amino acid biosynthetic genes. PMID: 21111745
    11. GAAC is a major effector of the TOR pathway, with Gcn4p and Gln3p each inducing a similar number of genes during rapamycin treatment PMID: 20233714
    12. interactions between the two nanorope-like assemblies are due to the hydrophobic residues on the leucine faces, which are also responsible for the dimer formation of the wild-type GCN4 leucine zipper. PMID: 20027625
    13. eIF3b functions between 48S assembly and subunit joining to influence AUG recognition and reinitiation on GCN4 mRNA PMID: 14976554
    14. Transcriptional activator Gcn4p recruits SAGA and Srb mediator to the upstream activation sequence of the promoter regions. PMID: 15831453
    15. Site-specific photocrosslinkers positioned within the central transcription-activating region of yeast Gcn4 were used to identify three polypeptides in direct physical proximity to the activator during the process of transcription activation. PMID: 15866178
    16. eIF5 is critical for the integrity of the scanning preinitiation complex and the accurate control of GCN4 translation. PMID: 15964804
    17. Simultaneous recruitment of trans-activators by GCN4 stimulates multiple steps of transcription in vivo. PMID: 15964818
    18. GCN4 expression is induced by sodium and has a role in controlling the accumulation of the 5' to 3' RNA degradation inhibitor, 3'-phosphoadenosine 5'-phosphate PMID: 16352596
    19. large fraction of genes activated by Gcn4p are repressed by H3 and H4 amino termini under non-inducing conditions, indicating that one function of Gcn4p is to overcome chromatin-mediated repression that depends on histone tails PMID: 16461773
    20. report here the design of a modified GCN4-bZIP DNA-binding protein with an azobenzene chromophore introduced between Cys residues at positions 262 and 269 (S262C, N269C) within the zipper domain PMID: 16681380
    21. a set of native or nativelike tertiary interactions, distributed throughout the entire sequence, collectively stabilize the folding transition state of the GCN4 coiled coil. PMID: 16953584
    22. Results describe the CPC1 regulon, the ortholog of Saccharomyces cerevisiae GCN4, by profiling transcription in wild-type and cpc-1 mutant strains. PMID: 17449655
    23. LZGCN4 x-form is generally less structured in comparison with the classical GCN4-p1 coiled coil, but still retains a structured alpha-helical central core PMID: 17469817
    24. Rpn13 protein and Rpn14 protein are involved in the efficient recognition of 26S proteasome for the proteolysis of ubiquitinated Gcn4 protein. PMID: 17499717
    25. Gcn4 is required for the response to peroxide stress in the yeast Saccharomyces cerevisiae PMID: 18417611
    26. Genetic epistasis analyses suggest that dietary restriction, reduced 60S subunit abundance, and Gcn4 activation extend yeast life span by similar mechanisms. PMID: 18423200
    27. Results suggest that decreasing cargo proteins in the late endosome through impaired delivery or enhanced degradation is required to attenuate substantially transcriptional activation by Gcn4. PMID: 18794364
    28. Adaptation to methylglyoxal was impaired in gcn4Delta cells, indicating the expression of certain genes regulated by Gcn4 to be important for the adaptive response to methylglyoxal. PMID: 18812164
    29. 5,764 mRNAs, 54 metabolites, and 83 experimental (13)C-based reaction fluxes were quantified in continuous cultures of yeast under stress in the absence or presence of global regulator Gcn4p. PMID: 19346491

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    BZIP family, GCN4 subfamily
  • 数据库链接：

    KEGG: sce:YEL009C

    STRING: 4932.YEL009C