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  4. Recombinant Saccharomyces cerevisiae H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1)

Recombinant Saccharomyces cerevisiae H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1)

  • 货号：
    CSB-YP015412SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015412SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015412SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015412SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015412SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NAF1
  • Uniprot No.：
  • 别名：
    NAF1; YNL124W; N1888; H/ACA ribonucleoprotein complex non-core subunit NAF1; Nuclear assembly factor 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-492
  • 氨基酸序列
    MSDDLFSKAL ENPDQDLNVE LPKDDVDLGL LGDGGNERKT DEPVADAERS TGLGSGSSES ESDSGSDSDS DSGSSGSEDD SADQDVEGED EGGDAIENED EDEDPSPSGP ILSKNEILEE TVPELPEDYE ISEKTIITPI GVLKSAFENN IIIHATMSGE KRVLKEGSIF CLEDRTLIGM LTEVFGPLQN PFYRIKLPDS KKNLFDELKV RLGEKAFIVT PDAHWIDTFE LKRNKGTDAS NGYDEELPEE EQEFSDDEKE ALFKKMKKQQ RQRKKRDNRK LANDSDNVKV KRARQPKANS LPKLVPPLGM SSNAPMQHGY KSRNARENIK RESSATSNRN GSSPVPITQH HQQQFSANNY PFPQQPNGMP YPPYSPFPQP TNFQYPPPPF GQATPAQFSN TVPYGSLPPA YNNMSPPTQQ SFMPMTQSQP PLPYGVPPMN QMQNPMYIQP PPQAPPQGNG NFQQVMELHQ ILLQQQQQQH QYQHQHQQDP RT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by GAR1 to yield mature H/ACA snoRNPs complex. Acts as a competitive binder for CBF5 probably required to prevent non-cognate RNAs from being loaded during transport of the particle by inducing a non-productive conformation of CBF5.
  • 亚细胞定位：
    Nucleus. Note=Mostly present in the nucleoplasm. Absent from the nucleolus.
  • 蛋白家族：
    NAF1 family
  • 数据库链接：

    KEGG: sce:YNL124W

    STRING: 4932.YNL124W