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  4. Recombinant Saccharomyces cerevisiae Histone H3 (HHT1)

Recombinant Saccharomyces cerevisiae Histone H3 (HHT1)

  • 货号：
    CSB-YP352255SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP352255SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP352255SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP352255SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP352255SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HHT1
  • Uniprot No.：
  • 别名：
    HHT1; YBR010W; YBR0201;; HHT2; SIN2; YNL031C; N2749; Histone H3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-136
  • 氨基酸序列
    ARTKQTARK STGGKAPRKQ LASKAARKSA PSTGGVKKPH RYKPGTVALR EIRRFQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAI GALQESVEAY LVSLFEDTNL AAIHAKRVTI QKKDIKLARR LRGERS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Component of the UAF (upstream activation factor) complex which interacts with the upstream element of the RNA polymerase I promoter and forms a stable preinitiation complex. Together with SPT15/TBP, UAF seems to stimulate basal transcription to a fully activated level.
  • 基因功能参考文献：
    1. A model has been proposed wherein combined actions of Set1 and Jhd2 via modulating H3K4 methylation-demethylation together control chromatin dynamics during various facets of transcriptional regulation. PMID: 27325136
    2. Data indicate histone post-translational modifications (PTMs) enriched at a DNA double-strand break (DSB) site. PMID: 29036368
    3. The mutant ion at Histone H3 doesn't affect the recruitment of Pol II repressor RENT (regulator of nucleolar silencing and telophase exit) complex at the Ribosomal DNA region. PMID: 26906758
    4. results suggest that divergent amino acids within the histone H3 alpha3 helix play organismal roles in defining chromatin structure PMID: 26534951
    5. Data show that vacuolar protein Prb1 is present in the cellular fraction enriched for the H3 N-terminus endopeptidase activity and this endopeptidase activity is lost in the PRB1 deletion mutant (prb1Delta). PMID: 24587380
    6. The molecular mechanism linking double strand break sequences to chromosome axes and the importance of H3K4 methylation for meiotic recombination. PMID: 23246437
    7. Studies identified mutations altering eight histone residues (H3 residues V46, R49, V117, Q120, and K122 and H4 residues R36, I46, and S47) that strongly increase SER3 expression without reducing the transcription of the intergenic SRG1 ncDNA. PMID: 21730290
    8. Data identify and characterize H3-T45 phosphorylation as a replication-associated histone modification in budding yeast. PMID: 20139971
    9. Histone H3 K56 acetylation at the entry-exit gate enables recruitment of the SWI/SNF nucleosome remodeling complex and so regulates gene activity. PMID: 15882620
    10. Investigation of kinetochore assembly in the absence of the centromeric histone H3 variant Cse4. PMID: 16207811
    11. Hst3 Is regulated by Mec1-dependent proteolysis and controls the S phase checkpoint and sister chromatid cohesion by deacetylating histone H3 at lysine 56 PMID: 17977840
    12. analysis of the molecular mechanism of the recognition and binding of the methylated H3K36 by Eaf3 provides new insights into the functional roles of the Eaf3 chromo domain PMID: 18984594
    13. Collectively, these results indicate that different histone H3 Arg2 methylation states have defined roles in gene expression. PMID: 19270702

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H3 family
  • 数据库链接：

    KEGG: sce:YBR010W

    STRING: 4932.YNL031C