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  4. Recombinant Saccharomyces cerevisiae Histone deacetylase RPD3 (RPD3)

Recombinant Saccharomyces cerevisiae Histone deacetylase RPD3 (RPD3)

  • 货号：
    CSB-YP335742SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP335742SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP335742SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP335742SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP335742SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RPD3
  • Uniprot No.：
  • 别名：
    RPD3; MOF6; REC3; SDI2; SDS6; YNL330C; N0305; Histone deacetylase RPD3; EC 3.5.1.98; Transcriptional regulatory protein RPD3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-433
  • 氨基酸序列
    MVYEATPFDP ITVKPSDKRR VAYFYDADVG NYAYGAGHPM KPHRIRMAHS LIMNYGLYKK MEIYRAKPAT KQEMCQFHTD EYIDFLSRVT PDNLEMFKRE SVKFNVGDDC PVFDGLYEYC SISGGGSMEG AARLNRGKCD VAVNYAGGLH HAKKSEASGF CYLNDIVLGI IELLRYHPRV LYIDIDVHHG DGVEEAFYTT DRVMTCSFHK YGEFFPGTGE LRDIGVGAGK NYAVNVPLRD GIDDATYRSV FEPVIKKIME WYQPSAVVLQ CGGDSLSGDR LGCFNLSMEG HANCVNYVKS FGIPMMVVGG GGYTMRNVAR TWCFETGLLN NVVLDKDLPY NEYYEYYGPD YKLSVRPSNM FNVNTPEYLD KVMTNIFANL ENTKYAPSVQ LNHTPRDAED LGDVEEDSAE AKDTKGGSQY ARDLHVEHDN EFY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic component of the RPD3 histone deacetylase (HDAC) complexes RPD3C(L) and RPD3C(S) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation plays an important role in transcriptional regulation, cell cycle progression, DNA damage response, osmotic stress response and developmental events. Is involved in rDNA and telomere silencing and in double strand breaks repair. Required for both full transcription repression and activation of many genes including cell type-specific genes (STE6, TY2 and HO), cell differentiation-specific genes (SPO13), genes that respond to external signals (PHO5) and TRK2. The RPD3 complexes regulate also chromosomal replication timing.
  • 基因功能参考文献：
    1. Deletion of RPD3 prevents cells from establishing transcriptional quiescence, leading to defects in quiescence entry and shortening of chronological lifespan. PMID: 26300265
    2. Data indicate that chromatin remodelers enhance Rpd3S activity by altering nucleosomal spacing. PMID: 24055344
    3. An important role for Rpd3 in promoting checkpoint adaptation via deacetylation and inhibition of Rad53. PMID: 23979600
    4. The Rpd3L histone deacetylase complex was required for diauxic shift-induced histone H4 and H2B deposition onto rDNA genes. PMID: 23689130
    5. Rpd3 core complex could contribute to repression via a novel nucleosome stabilization function. PMID: 22177115
    6. Mutation or inhibition of yeast Rpd3L or Hda1 suppressed up to 90% of CTG*CAG repeat expansions. PMID: 22363205
    7. Phosphorylated Pol II CTD recruits multiple HDACs, including Rpd3C(S), for methylation-dependent deacetylation of ORF nucleosomes. PMID: 20670892
    8. Rpd3 is the yeast ortholog of mammalian HDAC1. It activates transcription factor SBF. PMID: 19823669
    9. Raf60 is a component of the Rpd3 histone deacetylase complex and that it is required for normal Rpd3 complex activity and repression of gene expression PMID: 16275642
    10. Rpd3S plays an important role in Pol II-associated Set2 methylation of H3, providing a transcriptional memory which signals for deacetylation of open reading frames. PMID: 16286007
    11. The smaller complex, Rpd3C(S), was recruited by the chromodomain of Eaf3 to nucleosomes methylated by Set2 on histone H3 lysine 36, leading to deacetylation of transcribed regions. PMID: 16286008
    12. In this report, we identify by mass spectrometry and MudPIT the subunits of the Rpd3L complex, Two sequence-specific repressors, Ash1 and Ume6, were stably associated with Rpd3L PMID: 16314178
    13. RPD3 plays a role in the maintenance of an rRNA gene chromatin structure(s) that allows Pol II transcription of rRNA genes. PMID: 16648483
    14. Genetic experiments demonstrated that cells lacking the Rpd3S-specific subunits Eaf3 or Rco1 did not display the anti-silencing phenotype of mutations in SET2 or H3-K36. PMID: 17179083
    15. data provide evidence that during anaerobiosis, the Rpd3 complex acts at the DAN1 promoter to antagonize the chromatin-mediated repression caused by Mot3 and Rox1 and that chromatin remodeling by Swi/Snf is necessary for normal expression PMID: 17210643
    16. the coupled chromo and PHD domains of Rpd3S specify recognition of the methyl H3K36 mark, demonstrating the first combinatorial domain requirement within a protein complex to read a specific histone code PMID: 17510366
    17. Study identified the histone deacetylase Rpd3p as an attenuator of base composition-dependent differences in chromatin status. PMID: 17577398
    18. Results show that Rpd3(L) and Rpd3(S), distinct multisubunit complexes containing Rpd3 histone deacetylase, have distinct functions and the relative amounts of the 2 forms alter effectiveness of other chromatin-altering complexes, such as FACT and NuA4. PMID: 18490440
    19. disruption of histone deacetylase Rpd3p results in defective boundary activity, leading to a Sir-dependent local propagation of transcriptional repression. PMID: 19372273
    20. loss of Rpd3 function results in higher levels of histone H3 and H4 acetylation surrounding Rpd3-regulated origins PMID: 19417103
    21. Results implicate Rpd3p as an important co-factor in the Environmental Stress Response regulatory network, and suggest the importance of histone modification in producing transient changes in gene expression triggered by stress. PMID: 19470158

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Histone deacetylase family, HD type 1 subfamily
  • 数据库链接：

    KEGG: sce:YNL330C

    STRING: 4932.YNL330C