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  4. Recombinant Saccharomyces cerevisiae Meiosis-specific protein SPO11 (SPO11)

Recombinant Saccharomyces cerevisiae Meiosis-specific protein SPO11 (SPO11)

  • 中文名称：
    酿酒酵母SPO11重组蛋白
  • 货号：
    CSB-YP332895SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母SPO11重组蛋白
  • 货号：
    CSB-EP332895SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母SPO11重组蛋白
  • 货号：
    CSB-EP332895SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母SPO11重组蛋白
  • 货号：
    CSB-BP332895SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母SPO11重组蛋白
  • 货号：
    CSB-MP332895SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SPO11
  • Uniprot No.：
  • 别名：
    SPO11; YHL022CMeiosis-specific protein SPO11; EC 5.6.2.2; Sporulation-specific protein 11
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-398
  • 氨基酸序列
    MALEGLRKKY KTRQELVKAL TPKRRSIHLN SNGHSNGTPC SNADVLAHIK HFLSLAANSL EQHQQPISIV FQNKKKKGDT SSPDIHTTLD FPLNGPHLCT HQFKLKRCAI LLNLLKVVME KLPLGKNTTV RDIFYSNVEL FQRQANVVQW LDVIRFNFKL SPRKSLNIIP AQKGLVYSPF PIDIYDNILT CENEPKMQKQ TIFPGKPCLI PFFQDDAVIK LGTTSMCNIV IVEKEAVFTK LVNNYHKLST NTMLITGKGF PDFLTRLFLK KLEQYCSKLI SDCSIFTDAD PYGISIALNY THSNERNAYI CTMANYKGIR ITQVLAQNNE VHNKSIQLLS LNQRDYSLAK NLIASLTANS WDIATSPLKN VIIECQREIF FQKKAEMNEI DARIFEYK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The action of SPO11 is important in setting off a regulatory chain of events encompassing 5' to 3' resection. When there are no SPO11-DSBs, resection of a site specific VDE-DSB takes place but it is faster than in wild-type meiosis and increases the risk of uncovering flanking homology.
  • 基因功能参考文献：
    1. Data show that increases in meiotic double-strand breaks and crossover frequencies can be programmed to arbitrary genomic sites by fusion of the Spo11 protein to various DNA-recognition domains. PMID: 28977556
    2. These findings provide an explanation for the unexpectedly low prototroph levels exhibited by spo11 hypomorphs and have important implications for genetic studies that assume an unbiased recovery of prototrophs PMID: 24204324
    3. Study shows that Spo11-accessory proteins Rec114, Mer2, and Mei4 stably interact with chromosome axis sequences, upon phosphorylation of Mer2 by S phase Cdk. PMID: 21816273
    4. meiotic double-stranded breaks in budding yeast are processed by endonucleolytic cleavage that releases Spo11 attached to an oligonucleotide with a free 3'-OH PMID: 16107854
    5. Analysis of mutation sites of isolated spo11-mutant alleles indicated that both N-terminal and C-terminal non-conserved residues of Spo11 are essential for the protein's function. PMID: 16816949
    6. Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114 occurs at the time of DNA double-stranded break formation. PMID: 17264124
    7. N-terminal part of Spo11p is a nuclear localization signal that is not specific for prophase I and is used to import proteins in vegetative yeast cells. PMID: 18702308
    8. How to detect these Spo11-oligo complexes in extracts made from meiotic yeast cells. PMID: 19799183

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    TOP6A family
  • 数据库链接：

    KEGG: sce:YHL022C

    STRING: 4932.YHL022C