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  4. Recombinant Saccharomyces cerevisiae Mitochondrial import receptor subunit TOM22 (TOM22), partial

Recombinant Saccharomyces cerevisiae Mitochondrial import receptor subunit TOM22 (TOM22), partial

  • 货号：
    CSB-YP343000SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP343000SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP343000SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP343000SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP343000SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TOM22
  • Uniprot No.：
  • 别名：
    TOM22; MAS17; MAS22; MOM22; YNL131W; N1217; N1862; Mitochondrial import receptor subunit TOM22; Mitochondrial 17 kDa assembly protein; Mitochondrial 22 kDa outer membrane protein; Protein MAS17; Translocase of outer membrane 22 kDa subunit
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM70 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Docks TOM20 and TOM70 for interaction with the general TOM40 import pore (GIP) complex. May regulate the TOM machinery organization, stability and channel gating.
  • 基因功能参考文献：
    1. Tom22 is regulated by the cytosolic kinases CK2 and PKA and by membranebound CK1. PMID: 24093680
    2. Study shows that the cytosolic domain of Tom22 links the outer mitochondrial membrane and the sorting and assembly machinery into a supercomplex, facilitating precursor transfer on the intermembrane space side and reveals receptor-mediated coupling of import and export translocases as a means of precursor channeling. PMID: 23911324
    3. We also identified a core sequence of Tom22 that binds to Tim21. Furthermore, negatively charged amino-acid residues of Tom22 are important for binding to Tim21. Here we suggest a mechanism for the TOM-TIM interaction. PMID: 17099692
    4. Data Show that Tom22 are anchored in the outer membrane by a single transmembrane alpha-helix, located in the C-terminal portion of Tom22 (tail-anchored) and insertion of the precursor of Tom22 into the outer membrane requires pre-existing Tom receptors. PMID: 17974559
    5. beta2GP1-dependent uptake is mediated by bridging of the target cell to the phagocyte through the protein C- and N-terminal domains, respectively PMID: 18063580

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  • 亚细胞定位：
    Mitochondrion outer membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Tom22 family
  • 数据库链接：

    KEGG: sce:YNL131W

    STRING: 4932.YNL131W