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  4. Recombinant Saccharomyces cerevisiae N-acetyltransferase ECO1 (ECO1)

Recombinant Saccharomyces cerevisiae N-acetyltransferase ECO1 (ECO1)

  • 中文名称：
    酿酒酵母ECO1重组蛋白
  • 货号：
    CSB-YP337462SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母ECO1重组蛋白
  • 货号：
    CSB-EP337462SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母ECO1重组蛋白
  • 货号：
    CSB-EP337462SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母ECO1重组蛋白
  • 货号：
    CSB-BP337462SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母ECO1重组蛋白
  • 货号：
    CSB-MP337462SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ECO1
  • Uniprot No.：
  • 别名：
    ECO1; CTF7; YFR027WN-acetyltransferase ECO1; EC 2.3.1.-; Chromosome transmission fidelity protein 7; Establishment of cohesion protein 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-281
  • 氨基酸序列
    MKARKSQRKA GSKPNLIQSK LQVNNGSKSN KIVKCDKCEM SYSSTSIEDR AIHEKYHTLQ LHGRKWSPNW GSIVYTERNH SRTVHLSRST GTITPLNSSP LKKSSPSITH QEEKIVYVRP DKSNGEVRAM TEIMTLVNNE LNAPHDENVI WNSTTEEKGK AFVYIRNDRA VGIIIIENLY GGNGKTSSRG RWMVYDSRRL VQNVYPDFKI GISRIWVCRT ARKLGIATKL IDVARENIVY GEVIPRYQVA WSQPTDSGGK LASKYNGIMH KSGKLLLPVY I
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for establishment of sister chromatid cohesion during S phase but not for its further maintenance during G2 or M phases or for loading the cohesin complex onto DNA. Interacts with the three known alternate replication factor C (RFC) complexes, suggesting that these complexes have essential but redundant activity in cohesion establishment. Acts by acetylating the cohesin complex component SMC3. In vitro, possesses acetyltransferase activity where it can acetylate itself and components of the cohesin complex (MCD1, IRR1 and PDS5), but is unable to acetylate histones.
  • 基因功能参考文献：
    1. The s propose that the timing of Eco1 phosphorylation, and potentially that of other DDK substrates, is determined in part by sequestration of DDK at unfired replication origins during S phase. PMID: 28918948
    2. Eco1 mediated acetylation regulates PCNA sliding on DNA in the presence of DNA damage, favoring homologous recombination linked to sister-chromatid cohesion. PMID: 27916662
    3. Eco1p-mediated acetylation promotes establishment by abrogating the ability of Wpl1p to destabilize cohesin binding to chromosomes. PMID: 25378582
    4. Data indicate that cell cycle protein Pds5 promotes acetylation of cohesin subunit Smc3 by acetyltransferase Eco1 during S phase. PMID: 23878248
    5. the absence of Ctf4 and Chl1, but not of the other factors, causes a synthetic growth defect in cells lacking Eco1. PMID: 23334284
    6. Eco1 degradation requires the sequential actions of Cdk1 and two additional kinases, Cdc7-Dbf4 and the GSK-3 homolog Mck1. PMID: 23314252
    7. Acetylation of Mps3 by Eco1 is one of the few regulatory mechanisms known to control nuclear organization. PMID: 22593213
    8. Data suggest that Cdk1-dependent phosphorylation of Eco1 is a key factor limiting establishment to S phase. PMID: 21549314
    9. Eco1 promotes the reciprocal exchange of chromosome arms and maintenance of heterozygosity during mitosis. PMID: 20703090
    10. During chromatid cohesion establishment, Eco1 is found at the DNA replication fork. PMID: 16962805
    11. findings show that cohesion is generated by an Eco1-dependent but replication-independent mechanism in response to double-strand breaks in G(2)/M; Eco1 has 2 functions: a cohesive activity and a conserved acetyltransferase activity PMID: 17626885
    12. This study identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion. PMID: 18614053
    13. findings show that Smc3 is acetylated in an Eco1-dependent manner during DNA replication to promote sister chromatid cohesion PMID: 18653893
    14. study found that the head domain of the Smc3p subunit of cohesin is acetylated by the Eco1p acetyltransferase at two evolutionarily conserved residues, promoting the chromatin-bound cohesin to tether sister chromatids PMID: 18653894
    15. Cdc28p CDK acts in sister chromatid cohesion parallel to Ctf7p reactions PMID: 18716324
    16. required for the establishment of sister chromatid cohesion PMID: 18957238
    17. Results provide a model where Eco1p and Wpl1p act as a simple positive and negative regulatory switch controlling cohesion generation. PMID: 19450529
    18. The zinc finger may be a general enhancer of substrate recognition, thereby enhancing the ability of Eco1p to acetylate its substrates above a threshold needed to generate cohesion during DNA replication and repair. PMID: 19692582
    19. Eco1 regulates proteins in the cohesin network through acetylation and plays an important role in the subnuclear organization of chromatin. PMID: 19948494

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  • 亚细胞定位：
    Nucleus. Note=Associated with chromatin.
  • 蛋白家族：
    Acetyltransferase family, ECO subfamily
  • 数据库链接：

    KEGG: sce:YFR027W

    STRING: 4932.YFR027W