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  4. Recombinant Saccharomyces cerevisiae Neutral trehalase (NTH1), partial

Recombinant Saccharomyces cerevisiae Neutral trehalase (NTH1), partial

  • 中文名称：
    酿酒酵母NTH1重组蛋白
  • 货号：
    CSB-RP181594Ye(c)
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    NTH1
  • Uniprot No.：
  • 别名：
    NTH1; NTH; YDR001C; YD8119.07C; Neutral trehalase; EC 3.2.1.28; Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    27.6kDa
  • 表达区域：
    546-751aa
  • 氨基酸序列
    YMWDDESGFFFDYNTKIKHRTSYESATTFWALWAGLATKEQAQKMVEKALPKLEMLGGLAACTERSRGPISISRPIRQWDYPFGWAPHQILAWEGLRSYGYLTVTNRLAYRWLFMMTKAFVDYNGIVVEKYDVTRGTDPHRVEAEYGNQGADFKGAATEGFGWVNASYILGLKYMNSHARRALGACIPPISFFSSLRPQERNLYGL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Hydrolyzes intracellular trehalose to glucose (Probable). The disaccharide trehalose serves as a storage carbohydrate that is mobilized during nutrient stress (Probable). Regulates the level of trehalose as a protectant for cell integrity during heat stress.
  • 基因功能参考文献：
    1. EF-hand like motif-containing domain functions as the intermediary through which the 14-3-3 protein modulates the function of the catalytic domain of Nth1 PMID: 24713696
    2. 14-3-3 proteins are required for both activation of trehalase and phosphorylation of the ser21 and ser83 sites. PMID: 23155055
    3. Gene disruption of NTH1 and ATH1 increased tolerance to freezing, heat, high-sugar and ethanol concentration. PMID: 19160808
    4. The behaviour of trehalase mutant strains demonstrates the participation of trehalases Nth1p, Nth2p and Ath1p in the mobilization of intracellular trehalose during growth recovery after saline stress. PMID: 19520725
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Glycosyl hydrolase 37 family
  • 数据库链接：

    KEGG: sce:YDR001C

    STRING: 4932.YDR001C