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  4. Recombinant Saccharomyces cerevisiae Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1 (PUB1)

Recombinant Saccharomyces cerevisiae Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1 (PUB1)

  • 中文名称：
    酿酒酵母PUB1重组蛋白
  • 货号：
    CSB-YP339279SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母PUB1重组蛋白
  • 货号：
    CSB-EP339279SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母PUB1重组蛋白
  • 货号：
    CSB-EP339279SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母PUB1重组蛋白
  • 货号：
    CSB-BP339279SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母PUB1重组蛋白
  • 货号：
    CSB-MP339279SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PUB1
  • Uniprot No.：
  • 别名：
    PUB1; RNP1; YNL016W; N2842; Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1; ARS consensus-binding protein ACBP-60; Poly uridylate-binding protein; Poly(U)-binding protein
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-453
  • 氨基酸序列
    SENNEEQHQ QQQQQQPVAV ETPSAVEAPA SADPSSEQSV AVEGNSEQAE DNQGENDPSV VPANAITGGR ETSDRVLYVG NLDKAITEDI LKQYFQVGGP IANIKIMIDK NNKNVNYAFV EYHQSHDANI ALQTLNGKQI ENNIVKINWA FQSQQSSSDD TFNLFVGDLN VNVDDETLRN AFKDFPSYLS GHVMWDMQTG SSRGYGFVSF TSQDDAQNAM DSMQGQDLNG RPLRINWAAK RDNNNNNNYQ QRRNYGNNNR GGFRQYNSNN NNNMNMGMNM NMNMNMNNSR GMPPSSMGMP IGAMPLPSQG QPQQSQTIGL PPQVNPQAVD HIIRSAPPRV TTAYIGNIPH FATEADLIPL FQNFGFILDF KHYPEKGCCF IKYDTHEQAA VCIVALANFP FQGRNLRTGW GKERSNFMPQ QQQQGGQPLI MNDQQQPVMS EQQQQQQQQQ QQQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be associated with hnRNA within the nucleus and remains associated during nucleocytoplasmic mRNA transport, once the proteins are in the cytoplasm, disassembly of PUB1-RNA complexes may occur prior to PAB1 binding and formation of a translationally competent RNP complex. Binds to polyadenylated RNA; prefers to bind poly(rU); binds to T-rich single-stranded DNA.
  • 基因功能参考文献：
    1. Mutagenesis analysis revealed that residues on the beta-sheets of Pub1RRM1 and Pub1RRM2 are critical for poly(U) binding. PMID: 20438847
    2. Global analysis of Pub1 targets reveals a coordinate control of gene expression through modulation of binding and stability. PMID: 15964806
    3. PUB1 functions together with NAB2 to modulate mRNA stability and strengthen a model where nuclear events are coupled to the control of mRNA in the cytoplasm. PMID: 17636033
    4. The second of three RNA-recognition motifs (RRM) of Pub1 has been crystallized in this study; the second RRM may play a central role in poly(U) binding and determine the binding specificity of Pub1. PMID: 19193997
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 数据库链接：

    KEGG: sce:YNL016W

    STRING: 4932.YNL016W