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  4. Recombinant Saccharomyces cerevisiae Nuclear fusion protein BIK1 (BIK1)

Recombinant Saccharomyces cerevisiae Nuclear fusion protein BIK1 (BIK1)

  • 货号：
    CSB-YP320897SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP320897SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP320897SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP320897SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP320897SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    BIK1
  • Uniprot No.：
  • 别名：
    BIK1; YCL029C; YCL29CNuclear fusion protein BIK1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-440
  • 氨基酸序列
    MDRYQRKIGC FIQIPNLGRG QLKYVGPVDT KAGMFAGVDL LANIGKNDGS FMGKKYFQTE YPQSGLFIQL QKVASLIEKA SISQTSRRTT MEPLSIPKNR SIVRLTNQFS PMDDPKSPTP MRSFRITSRH SGNQQSMDQE ASDHHQQQEF GYDNREDRME VDSILSSDRK ANHNTTSDWK PDNGHMNDLN SSEVTIELRE AQLTIEKLQR KQLHYKRLLD DQRMVLEEVQ PTFDRYEATI QEREKEIDHL KQQLELERRQ QAKQKQFFDA ENEQLLAVVS QLHEEIKENE ERNLSHNQPT GANEDVELLK KQLEQLRNIE DQFELHKTKW AKEREQLKMH NDSLSKEYQN LSKELFLTKP QDSSSEEVAS LTKKLEEANE KIKQLEQAQA QTAVESLPIF DPPAPVDTTA GRQQWCEHCD TMGHNTAECP HHNPDNQQFF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for nuclear fusion, chromosome disjunction, and nuclear segregation during mitosis. Probably required for the formation or stabilization of microtubules during mitosis and for spindle pole body fusion during conjugation.
  • 基因功能参考文献：
    1. the microtubule-associated proteins Bik1 and Bim1 are important host factors in the process of plasmid localization to a certain sub-nuclear locale probably assisted by the motor protein, Kip1 and microtubules, perhaps by acting as an adapter between the plasmid and the motor and thus helping to anchor the plasmid to microtubules. PMID: 28004422
    2. Bik1p promotes the phosphorylation of Kar9p on serine 496, which affects its asymmetric localization to one SPB and associated cytoplasmic microtubules PMID: 16236795
    3. Bik1p has a previously uncharacterized function in the separation of duplicated Spindle Pole Bodies. PMID: 16972090
    4. functions of Bik1p and the various roles that Bik1p plays in positioning the mitotic spindle PMID: 17118263
    5. These results indicate that Bik1p interactions with tubulin are important for Bik1p plus-end tracking, and suggest alternative pathways for Bik1p-Kip2p-dependent dynein localization at plus ends. PMID: 18411245

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Note=And mitotic spindle.
  • 数据库链接：

    KEGG: sce:YCL029C

    STRING: 4932.YCL029C