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  4. Recombinant Saccharomyces cerevisiae PHO85 cyclin-1 (PCL1)

Recombinant Saccharomyces cerevisiae PHO85 cyclin-1 (PCL1)

  • 货号：
    CSB-YP328505SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP328505SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP328505SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP328505SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP328505SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PCL1
  • Uniprot No.：
  • 别名：
    PCL1; HCS26; YNL289W; N0536PHO85 cyclin-1; Cyclin HCS26; G1/S-specific cyclin PCL1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-279
  • 氨基酸序列
    MCEYSKALHI LLKSPVTDDI IKFLTDTTLR VVPSSNYPTP PGSPGEKHLT RLPSLMTFIT RLVRYTNVYT PTLLTAACYL NKLKRILPRD ATGLPSTIHR IFLACLILSA KFHNDSSPLN KHWARYTDGL FTLEDINLME RQLLQLLNWD LRVNTEDLIL DLQPLLEPIK QDLARSSDQR KRINMMMSMN RRTCAGTSPI RSNNRFKLYE KQRNVSIASD LSSATLVDSC NDLRRLKDVT NIANNTVANT NYVRTVEKWN DNVNRQSWDL EQIMSQHGF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    G1/S-specific cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Essential for the control of the cell cycle at the G1/S (start) transition. The PCL1-PHO85 cyclin-CDK holoenzyme is involved in phosphorylation of the CDK inhibitor (CKI) SIC1, which is required for its ubiquitination and degradation, releasing repression of b-type cyclins and promoting exit from mitosis. Together with cyclin PCL2, positively controls degradation of sphingoid long chain base kinase LCB4. PCL1-PHO85 phosphorylates LCB4, which is required for its ubiquitination and degradation. PCL1-PHO85 also phosphorylates HMS1, NCP1 and NPA3, which may all have a role in mitotic exit.
  • 基因功能参考文献：
    1. These results, together with a genetic correlation analysis, strongly support a functional model linking Slt2 MAP kinase and Pcl1, a Pho85 cyclin-dependent kinase, in septum assembly through Bni4. PMID: 27400980
  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Localizes to the incipient bud site.
  • 蛋白家族：
    Cyclin family, PCL1,2 subfamily
  • 数据库链接：

    KEGG: sce:YNL289W

    STRING: 4932.YNL289W