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  4. Recombinant Saccharomyces cerevisiae Postreplication repair E3 ubiquitin-protein ligase RAD18 (RAD18)

Recombinant Saccharomyces cerevisiae Postreplication repair E3 ubiquitin-protein ligase RAD18 (RAD18)

  • 货号：
    CSB-YP319938SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP319938SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP319938SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP319938SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP319938SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RAD18
  • Uniprot No.：
  • 别名：
    RAD18; YCR066W; YCR66W; Postreplication repair E3 ubiquitin-protein ligase RAD18; EC 2.3.2.27; RING-type E3 ubiquitin transferase RAD18; Radiation sensitivity protein 18
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-487
  • 氨基酸序列
    MDHQITTASD FTTTSIPSLY QLDTLLRCHI CKDFLKVPVL TPCGHTFCSL CIRTHLNNQP NCPLCLFEFR ESLLRSEFLV SEIIQSYTSL RSSLLDALRI PKPTPVPENE EVPGPENSSW IELISESESD SVNAADDDLQ IVATSERKLA KRSMTDILPL SSKPSKRNFA MFRSERIKKK SKPNEQMAQC PICQQFYPLK ALEKTHLDEC LTLQSLGKKP KISTTFPTES NPHNKSSSRF KVRTPEVDKS SCGETSHVDK YLNSMMSAEH QRLPKINFTS MTQSQIKQKL SSLGLSTNGT RQNMIKRYNH YEMLWNSNFC DSLEPVDEAE LKRQLLSWDV SHNKTPQNSS NKGGISKLMI MKSNGKSSSY RKLLENFKND KFNRKGWMVM FRKDFARLIR EAKMKIKTGS SDSSGSVGHS NDGDGVEKVQ SDQGTEDQQM EKDQDTVINE DRVAGERNLP NEDSTDADLS RELMDLNEYS KDPPGNN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 RING-finger protein, member of the UBC2/RAD6 epistasis group. Associates to the E2 ubiquitin conjugating enzyme UBC2/RAD6 to form the UBC2-RAD18 ubiquitin ligase complex involved in postreplicative repair (PRR) of damaged DNA. The UBC2-RAD18 complex cooperates with RAD5 and the UBC13-MMS2 dimer to attach mono-ubiquitin chains on 'Lys-164' of POL30, which is necessary for PRR. The UBC2-RAD18 complex is also involved in prevention of spontaneous mutations caused by 7,8-dihydro-8-oxoguanine.
  • 基因功能参考文献：
    1. The Pol30-K196 residue plays a critical role in budding yeast DNA post-replication repair through interaction with Rad18. PMID: 27707542
    2. following UV-induced damage, reactivation of Rad18, even after prolonged G2 arrest, restores viability and genome integrity. PMID: 23382077
    3. The results imply that sumoylated PCNA is the physiological ubiquitylation target of budding yeast Rad18. PMID: 23034805
    4. In this study we showed that DNA damage induced by hydrogen peroxide at the same mutagen doses causes significantly more mutations and lethal events in the rad18 mutant cells compared to control wild-type cells PMID: 22730775
    5. Rad17, a subunit of the PCNA-like 9-1-1 checkpoint clamp is ubiquitylated; however, in contrast to previous results, modification of Rad17 is found to be independent of DNA damage, the Rad6-Rad18 complex. PMID: 20550940
    6. Rad6-Rad18 and Ubc13-Mms2-Rad5 act sequentially and mediate proliferating cell nuclear antigen poly-ubiquitylation by stepwise addition of ubiquitin monomers. PMID: 19851286
    7. role in polyubiquitination of PCNA PMID: 15507115
    8. error-free component of the RAD6/RAD18 pathway completes replication by a mechanism employing recombination between partially replicated sister strands, possibly by means of transient template strand switching or copy choice. PMID: 16247017
    9. Study suggests that Rad18/Rad5/Mms2 and Mgs1 are required to promote replication when forks are destabilized or stalled due to defects in Pol delta. PMID: 16949354
    10. Rad6-Rad18 promotes DNA-damage-dependent transcriptional induction as well as checkpoint functions by catalyzing monoubiquitination at the K197 residue of the Rad17 subunit of the 9-1-1 complex. PMID: 18485869
    11. The interactions between Rad18, RPA and DNA and discuss their contribution to the activation of DNA damage bypass, was examined. PMID: 19029798
    12. key role for the RAD6-RAD18-RAD5 error-free postreplication repair (RAD6 error-free PRR) pathway in promoting cell growth and survival, in response to chronic low-dose ultraviolet light PMID: 19079240
    13. Rad18 is required for the formation of X-shaped sister chromatid junctions (SCJs) at damaged replication forks through a process involving PCNA polyubiquitylation and the ubiquitin-conjugating enzymes Mms2 and Ubc13 PMID: 19092928

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RAD18 family
  • 数据库链接：

    KEGG: sce:YCR066W

    STRING: 4932.YCR066W