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  4. Recombinant Saccharomyces cerevisiae Pre-60S factor REI1 (REI1)

Recombinant Saccharomyces cerevisiae Pre-60S factor REI1 (REI1)

  • 中文名称：
    酿酒酵母REI1重组蛋白
  • 货号：
    CSB-YP339955SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母REI1重组蛋白
  • 货号：
    CSB-EP339955SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母REI1重组蛋白
  • 货号：
    CSB-EP339955SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母REI1重组蛋白
  • 货号：
    CSB-BP339955SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母REI1重组蛋白
  • 货号：
    CSB-MP339955SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    REI1
  • Uniprot No.：
  • 别名：
    REI1; YBR267W; YBR1736; Cytoplasmic 60S subunit biogenesis factor REI1; Required for isotropic bud growth protein 1; pre-60S factor REI1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-393
  • 氨基酸序列
    MSSSGVYTCN SCVLTFDSSD EQRAHMKSDW HRYNLKRRVA QLPPISFETF DSKVSAAAAS TSKSAEKEKP VTKKELKRRE KQALLEKKKK LLEIARANML ENMQKSQEGN TPDLSKLSLQ ENEENKEKEE PKKEEPEQLT EEEMAERVMQ EKLRNRVDIP LEQCLFCEHN KHFKDVEENL EHMFRTHGFY IPEQKYLVDK IGLVKYMSEK IGLGNICIVC NYQGRTLTAV RQHMLAKRHC KIPYESEDER LEISEFYDFT SSYANFNSNT TPDNEDDWED VGSDEAGSDD EDLPQEYLYN DGIELHLPTG IKVGHRSLQR YYKQDLKPEV ILTEGQGTLV AAETRSFLPA FDKKGVQTQQ RVWQTERFDK KRLDKRSAKF VNNQPHYRDQ LLQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Pre-60S-associated factor involved in the cytoplasmic maturation of the 60S subunit. Involved in the dissociation and recycling of other late pre-60S factors like ARX1, TIF6 and ALB1 before newly synthesized large ribosomal subunits enter translation. Cooperates with the co-chaperone JJJ1 in the release of the nuclear-export factor ARX1. May act redundantly with REH1 to directly promote a stabilizing structural rearrangement in cytoplasmic 60S subunit maturation independent on ARX1 recycling.
  • 基因功能参考文献：
    1. Study reports the cryo-electron microscopy (cryo-EM) structure of the yeast 60S subunit in complex with the biogenesis factors Rei1, Arx1, and Alb1 at 3.4 A resolution; provides genetic and biochemical evidence that failure to insert the C terminus of Rei1 precludes subsequent steps of 60S maturation. PMID: 26709046
    2. biochemically characterized ribosomal complexes of the yeast 60S-biogenesis factor Arx1 and late-maturation factors Rei1 and Jjj1 and determined their cryo-EM structures PMID: 23142985
    3. The REI1 is a new member of genes belonging to the mitotic signaling network that negatively regulates Swe1 kinase. PMID: 15107529
    4. Nuclear recycling of the pre-60S ribosomal subunit associated factor Arx1 depends on Rei1. PMID: 16648468
    5. A model is presented in which Rei1 is a key factor for the coordinated dissociation and recycling of the last pre-60S factors before newly synthesized large ribosomal subunits enter translation. PMID: 16651379
    6. Preferentially involved in the recycling of ribosomal pre-60S factors Arx1 and Alb1. PMID: 17652132

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    REI1 family
  • 数据库链接：

    KEGG: sce:YBR267W

    STRING: 4932.YBR267W