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  4. Recombinant Saccharomyces cerevisiae Pre-mRNA polyadenylation factor FIP1 (FIP1)

Recombinant Saccharomyces cerevisiae Pre-mRNA polyadenylation factor FIP1 (FIP1)

  • 中文名称：
    酿酒酵母FIP1重组蛋白
  • 货号：
    CSB-YP332788SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母FIP1重组蛋白
  • 货号：
    CSB-EP332788SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母FIP1重组蛋白
  • 货号：
    CSB-EP332788SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母FIP1重组蛋白
  • 货号：
    CSB-BP332788SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母FIP1重组蛋白
  • 货号：
    CSB-MP332788SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FIP1
  • Uniprot No.：
  • 别名：
    FIP1; YJR093C; J1911Pre-mRNA polyadenylation factor FIP1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-327
  • 氨基酸序列
    MSSSEDEDDK FLYGSDSELA LPSSKRSRDD EADAGASSNP DIVKRQKFDS PVEETPATAR DDRSDEDIYS DSSDDDSDSD LEVIISLGPD PTRLDAKLLD SYSTAATSSS KDVISVATDV SNTITKTSDE RLITEGEANQ GVTATTVKAT ESDGNVPKAM TGSIDLDKEG IFDSVGITTI DPEVLKEKPW RQPGANLSDY FNYGFNEFTW MEYLHRQEKL QQDYNPRRIL MGLLSLQQQG KLNSANDTDS NLGNIIDNNN NVNNANMSNL NSNMGNSMSG TPNPPAPPMH PSFPPLPMFG SFPPFPMPGM MPPMNQQPNQ NQNQNSK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Polymerase-regulating component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase PAP1. This inhibits the extension of an oligo(A) primer by limiting access of the RNA substrate to the C-terminal RNA binding domain of PAP1. Seems to tether PAP1 to the cleavage factor I.
  • 基因功能参考文献：
    1. these results indicate that the Fip1 linker, through its flexibility and protein/protein interactions, allows Pap1 to reach the 3' end of the cleaved RNA and efficiently initiate poly(A) addition. PMID: 21282348
    2. Data elucidate the role of Fip1 in the mRNA 3'-OH polyadenylation process. PMID: 16496213
    3. in the absence of Pap1, Fip1 is largely, if not completely, unfolded PMID: 18537269
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    FIP1 family
  • 数据库链接：

    KEGG: sce:YJR093C

    STRING: 4932.YJR093C