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  4. Recombinant Saccharomyces cerevisiae Protein KES1 (KES1)

Recombinant Saccharomyces cerevisiae Protein KES1 (KES1)

  • 中文名称：
    酿酒酵母KES1重组蛋白
  • 货号：
    CSB-YP336093SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母KES1重组蛋白
  • 货号：
    CSB-EP336093SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母KES1重组蛋白
  • 货号：
    CSB-EP336093SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母KES1重组蛋白
  • 货号：
    CSB-BP336093SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母KES1重组蛋白
  • 货号：
    CSB-MP336093SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KES1
  • Uniprot No.：
  • 别名：
    KES1; OSH4; YPL145C; LPI3C; P2614Oxysterol-binding protein homolog 4; Protein KES1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-434
  • 氨基酸序列
    MSQYASSSSW TSFLKSIASF NGDLSSLSAP PFILSPISLT EFSQYWAEHP ELFLEPSFIN DDNYKEHCLI DPEVESPELA RMLAVTKWFI STLKSQYCSR NESLGSEKKP LNPFLGELFV GKWENKEHPE FGETVLLSEQ VSHHPPVTAF SIFNDKNKVK LQGYNQIKAS FTKSLMLTVK QFGHTMLDIK DESYLVTPPP LHIEGILVAS PFVELEGKSY IQSSTGLLCV IEFSGRGYFS GKKNSFKARI YKDSKDSKDK EKALYTISGQ WSGSSKIIKA NKKEESRLFY DAARIPAEHL NVKPLEEQHP LESRKAWYDV AGAIKLGDFN LIAKTKTELE ETQRELRKEE EAKGISWQRR WFKDFDYSVT PEEGALVPEK DDTFLKLASA LNLSTKNAPS GTLVGDKEDR KEDLSSIHWR FQRELWDEEK EIVL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1 phosphatase in the endoplasmic reticulum. Displays a similar affinity for PI4P and sterols. Binds sterol and PI4P in a mutually exclusive manner (Probable).
  • 基因功能参考文献：
    1. the sterol content in the mitochondrial fraction was significantly decreased in vivo after Osh4 inactivation in a genetic background in which all the other OSH genes were deleted. PMID: 29487131
    2. Osh4-mediated exocytosis depends upon lipid binding and exchange without a strict requirement for sterol. PMID: 28993464
    3. Osh4p transports sterol against its gradient between two membranes by dissipating the energy of a phosphatidylinositol-4-phosphate gradient. PMID: 25849868
    4. Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature. PMID: 25165144
    5. X-ray crystallographic determination of the conformational changes Osh4 undergoes during ligand binding. PMID: 23756172
    6. Kes1 (Osh4p) associated with exocytic vesicles that move from the mother cell into the bud. PMID: 21819498
    7. Study reports that Kes1 integrates multiple aspects of lipid metabolism in distal stages of the secretory pathway with TORC1 and nitrogen signaling. PMID: 22341443
    8. The structure of the Osh4p-phosphatidylinositol 4-phosphate complex was solved. It was shown that Osh4p quickly exchanges dehydroergosterol for phosphatidylinositol 4-phosphate. PMID: 22162133
    9. structure of the open state of a lid-truncated form of Osh4 was determined at 2.5 A resolution PMID: 16136145
    10. Osh4p/Kes1p, specifically facilitates the nonvesicular transfer of cholesterol and ergosterol between the plasma membrane and endoplasmic reticulum in vitro PMID: 16585271
    11. Kes1p regulates Golgi apparatus phosphatidylinositol-4-phosphate function PMID: 17881569
    12. The mechanism used by Osh4 to release cholesterol is conceptualized as a molecular ladder with the rungs being amino acids or water-mediated amino acids that interact with 3-hydroxyl group of cholesterol PMID: 18937371

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  • 亚细胞定位：
    Golgi apparatus membrane.
  • 蛋白家族：
    OSBP family
  • 数据库链接：

    KEGG: sce:YPL145C

    STRING: 4932.YPL145C