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  4. Recombinant Saccharomyces cerevisiae Protein RIF2 (RIF2)

Recombinant Saccharomyces cerevisiae Protein RIF2 (RIF2)

  • 货号：
    CSB-YP312340SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP312340SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP312340SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP312340SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP312340SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RIF2
  • Uniprot No.：
  • 别名：
    RIF2; YLR453C; Protein RIF2; RAP1-interacting factor 2
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-395
  • 氨基酸序列
    MEHVDSDFAP IRRSKKVVDS DKIVKAISDD LEQKNFTVLR KLNLVPIKKS VSSPKVCKPS PVKERVDHVF YQKFKSMALQ ELGTNYLSIS YVPSLSKFLS KNLRSMKNCI VFFDKVEHIH QYAGIDRAVS ETLSLVDINV VIIEMNDYLM KEGIQSSKSK ECIESMGQAS YSGQLDFEAS EKPSNHTSDL MMMVMRKINN DESIDHIVYF KFEQLDKLST STIIEPSKLT EFINVLSVLE KSNNIAFKVL IYSNNVSISS LLSTSLKKKL NTKYTVFEMP ILTCAQEQEY LKKMIKFTFD SGSKLLQSYN SLVTCQLNNK ESNLAIFFEF LKVFPHPFTY LFNAYTEIIV QSRTFDELLD KIRNRLTIKN YPHSAYNFKK NQRLPLKLTR KVHDR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in transcriptional silencing and telomere length regulation. Its role in telomere length regulation results from either a block in elongation or promoting degradation of the telomere ends. Loss of RIF1 function results in derepression of an HMR silencer, whose ARS consensus element has been deleted, and in the elongation of telomeres. RAP1 may target the binding of RIF1 to silencers and telomeres.
  • 基因功能参考文献：
    1. We identified an N-terminal domain in Rif2 that is essential for length regulation, which we have termed BAT domain for Blocks Addition of Telomeres PMID: 26294668
    2. The role of Tel1 in promoting MRX accumulation to double-strand breaks (DSBs)is counteracted by Rif2, which is recruited to DSBs. We also found that Rif2 enhances ATP hydrolysis by MRX and attenuates MRX function in end-tethering. PMID: 26901759
    3. The X-ray structures of Rif1 and Rif2 bound to the Rap1 C-terminal domain and that of the Rif1 C terminus are presented. Both Rif1 and Rif2 have separable and independent Rap1-binding epitopes, allowing Rap1 binding over large distances (42-110 A). PMID: 23746845
    4. The telomeric-bound protein, Rif2, was also found to promote a telomere fold-back through the recruitment of Rpd3L to telomeres PMID: 23028367
    5. Rif2 is unable to negatively regulate processing and lengthening at TEL1-hy909 telomeres, indicating that the Tel1-hy909 variant overcomes the inhibitory activity exerted by Rif2 on Mre11-Rad50-Xrs2 PMID: 22354991
    6. Data show that DNA ends that are protected by Yku and Rif2 involves multiple functions of the MRX complex that do not necessarily require its nuclease activity. PMID: 21152442
    7. Data show that the Rap1 C-terminal domain establishes two parallel inhibitory pathways through the proteins Rif2 and Sir4. PMID: 18451106

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  • 亚细胞定位：
    Nucleus. Chromosome, telomere.
  • 数据库链接：

    KEGG: sce:YLR453C

    STRING: 4932.YLR453C