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  4. Recombinant Saccharomyces cerevisiae Protein SIC1 (SIC1)

Recombinant Saccharomyces cerevisiae Protein SIC1 (SIC1)

  • 货号：
    CSB-YP327865SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP327865SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP327865SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP327865SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP327865SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SIC1
  • Uniprot No.：
  • 别名：
    SIC1; SDB25; YLR079W; L9449.8; Protein SIC1; CDK inhibitor p40
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-284
  • 氨基酸序列
    MTPSTPPRSR GTRYLAQPSG NTSSSALMQG QKTPQKPSQN LVPVTPSTTK SFKNAPLLAP PNSNMGMTSP FNGLTSPQRS PFPKSSVKRT LFQFESHDNG TVREEQEPLG RVNRILFPTQ QNVDIDAAEE EEEGEVLLPP SRPTSARQLH LSLERDEFDQ THRKKIIKDV PGTPSDKVIT FELAKNWNNN SPKNDARSQE SEDEEDIIIN PVRVGKNPFA SDELVTQEIR NERKRAMLRE NPDIEDVITY VNKKGEVVEK RRLTDEEKRR FKPKALFQSR DQEH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Substrate and inhibitor of the cyclin-dependent protein kinase CDC28. Its activity could be important for faithful segregation of chromosomes to daughter cells. It acts in response to a signal from a post-start checkpoint.
  • 基因功能参考文献：
    1. Studies propose that Sic1, Cdc37, and Hsp82 may orchestrate parts of the cellular starvation response by regulating transcription factor- and kinase activities. PMID: 24909858
    2. Data indicate that Sic1 function in analogy to its human counterpart p27(Kip1), whose deregulation leads to failure in timing of kinase activation and, therefore, to cancer. PMID: 22161326
    3. Ime2 does not directly catalyze Sic1 phosphorylation to target it for destabilization as Cln1,-2/Cdk1 do during the cell cycle PMID: 22393365
    4. We analyse oscillations of B-type (Clb) cyclins, focusing on the role of their inhibitor Sic1. PMID: 21963604
    5. Individually weak dispersed Sic1 phospho sites engage Cdc4 in a dynamic equilibrium. The multisite nature of this interaction confers cooperative dependence on kinase activity for Sic1 recognition & ubiquitination at equilibrium. PMID: 22328159
    6. Sic1 regulates waves of Clbs, acting as a timer in their appearance, thus controlling Cdk1-Clbs activation. The molecular mechanism is not yet fully understood but is hypothesized to occur via stoichiometric binding of Sic1 to Cdk1-Clb complexes. PMID: 22002907
    7. Computational simulation revealed that an increased amount of SIC1 mRNAs lead to an amplified dispersion of Sic1 protein levels, suggesting mRNA control being critical to set timing of Sic1 downregulation and, therefore, S phase onset PMID: 21717009
    8. we discovered previously unrecognized levels of regulation of the Ace2 transcription factor and the cyclin-dependent protein kinase inhibitor Sic1 PMID: 21196523
    9. CK2 plays a role in the regulation of Sic1 activity. PMID: 16168390
    10. These data indicate a previously unrecognized inhibitor/activator dual role for Sic1 and put it among key molecules whose activity is regulated by their nuclear-cytoplasmic localization. PMID: 16294029
    11. Ime2 is necessary but not sufficient to promote Sic1 destruction during sporulation. PMID: 16776651
    12. CK2 phosphorylates Sic1 in vivo PMID: 16777072
    13. Sic1 phosphorylation provides a sharp link between G(1) cyclin activation and Clb kinase activation, but failure of Sic1 phosphorylation and proteolysis imposes a variable cell cycle delay and extreme sensitivity to B-type cyclin dosage PMID: 17483408
    14. Here we show that CK2 inactivation up-regulates Sic1 level resulting in severe down-regulation of Clb5-Cdc28 kinase activity. PMID: 17574209
    15. Accumulation of SIC1 mutated at multiple phosphorylation sites (stabilized version) led to significant DNA rereplication in the absence of normal chromosome segregation PMID: 19116279
    16. Data concluded that the molecule exists in a highly disordered state but displays some content in secondary and tertiary structure. The mutations S201A and S201E do not have significant effects on the conformational properties of the pure protein. PMID: 19280601

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 数据库链接：

    KEGG: sce:YLR079W

    STRING: 4932.YLR079W