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  4. Recombinant Saccharomyces cerevisiae Protein STE50 (STE50)

Recombinant Saccharomyces cerevisiae Protein STE50 (STE50)

  • 货号：
    CSB-YP328531SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP328531SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP328531SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP328531SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP328531SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    STE50
  • Uniprot No.：
  • 别名：
    STE50; YCL032W; YCL32W; Protein STE50
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-346
  • 氨基酸序列
    MEDGKQAINE GSNDASPDLD VNGTILMNNE DFSQWSVDDV ITWCISTLEV EETDPLCQRL RENDIVGDLL PELCLQDCQD LCDGDLNKAI KFKILINKMR DSKLEWKDDK TQEDMITVLK NLYTTTSAKL QEFQSQYTRL RMDVLDVMKT SSSSSPINTH GVSTTVPSSN NTIIPSSDGV SLSQTDYFDT VHNRQSPSRR ESPVTVFRQP SLSHSKSLHK DSKNKVPQIS TNQSHPSAVS TANTPGPSPN EALKQLRASK EDSCERILKN AMKRHNLADQ DWRQYVLVIC YGDQERLLEL NEKPVIIFKN LKQQGLHPAI MLRRRGDFEE VAMMNGSDNV TPGGRL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in growth arrest during conjugation. May interact with the G protein alpha subunit.
  • 基因功能参考文献：
    1. Dynamic regulation of Ste50-Opy2 interaction fine-tunes the MAPK signaling network. PMID: 20932477
    2. Two peptide motifs within the intrinsically disordered cytoplasmic tail of Opy2p physically interact with the Step50p-RA domain. PMID: 19846660
    3. Results describe the solution structure of the sterile alpha motifs (SAM) domain of Ste11 and its interactions with the cognate SAM domain-containing region of Ste50. PMID: 15544813
    4. Yeast bearing mutations that prevented the heterotypic Ste11-Ste50 association in vitro presented signaling defects in the mating and high-osmolarity growth pathways PMID: 16337230
    5. Ste50 serves as an adaptor to tether Ste11 to the plasma membrane. PMID: 16428446
    6. propose that Opy2p can serve as a membrane anchor for the Ste50p/Ste11p module in the activation of the HOG pathway PMID: 16543225
    7. inactivation of STE50 influences stress tolerance in mutants of the Ras-cAMP pathway, which is a major determinant of intrinsic stress tolerance and cell survival PMID: 17318632
    8. Ste50 has a role in control of MAPK specificity by feedback phosphorylation PMID: 18854322

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  • 数据库链接：

    KEGG: sce:YCL032W

    STRING: 4932.YCL032W