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  4. Recombinant Saccharomyces cerevisiae Transcription initiation factor IIB (SUA7)

Recombinant Saccharomyces cerevisiae Transcription initiation factor IIB (SUA7)

  • 货号：
    CSB-YP010001SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010001SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010001SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010001SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010001SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SUA7
  • Uniprot No.：
  • 别名：
    SUA7; YPR086W; P9513.4; Transcription initiation factor IIB; General transcription factor TFIIB; Transcription factor E
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-345
  • 氨基酸序列
    MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDGN NLSTRIGKGE TTDMRFTKEL NKAQGKNVMD KKDNEVQAAF AKITMLCDAA ELPKIVKDCA KEAYKLCHDE KTLKGKSMES IMAASILIGC RRAEVARTFK EIQSLIHVKT KEFGKTLNIM KNILRGKSED GFLKIDTDNM SGAQNLTYIP RFCSHLGLPM QVTTSAEYTA KKCKEIKEIA GKSPITIAVV SIYLNILLFQ IPITAAKVGQ TLQVTEGTIK SGYKILYEHR DKLVDPQLIA NGVVSLDNLP GVEKK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
  • 基因功能参考文献：
    1. crystal structure of RNA polymerase II-TFIIB complex at 3.8 A resolution revealing carboxyl-terminal region of TFIIB, located above polymerase active center cleft but showing none of B finger; linker between amino- & carboxyl-terminal regions also seen PMID: 19965383
    2. Results describe the architecture of transcription factor IIB and the the RNA polymerase II transcription preinitiation complex (PIC). PMID: 15479635
    3. TBP1(E186D) temperature-sensitivity and slow-growth phenotypes might have been caused by insufficient amounts of Tbp1p for efficient transcription of the rRNA genes by RNA polymerase I PMID: 17680779
    4. TFIIB binds RNA Polymerase II at the terminator, which in turn associates with the promoter scaffold giving TFIIB a role in gene looping. PMID: 17803944
    5. the crystal structure of the complete RNA polymerase II - transcription factor IIB complex at 4.3 A resolution PMID: 19820686

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TFIIB family
  • 数据库链接：

    KEGG: sce:YPR086W

    STRING: 4932.YPR086W