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  4. Recombinant Saccharomyces cerevisiae Twinfilin-1 (TWF1)

Recombinant Saccharomyces cerevisiae Twinfilin-1 (TWF1)

  • 货号：
    CSB-YP025356SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025356SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025356SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025356SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025356SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TWF1
  • Uniprot No.：
  • 别名：
    TWF1; YGR080WTwinfilin-1; Twinfilin-A
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-332
  • 氨基酸序列
    MSTQSGIVAE QALLHSLNEN LSADGIVIII AKISPDSTSV HQTQVARSFE ELVQLASQER EPLYIFYKPE GLDKYFFVSF IPDGSPVRSR MLYASTKNTL ARQVGSNSLS TEQPLITDAQ DLVDLKNFDS ARPAGQNKPL THDEEMQIEI NKQQALLRKN TSVKLVSQDS ASPLSLTFRV NSEKPINEIL DSEGKNLIIF QIDPSNETIQ IVQSDTCPSV DELYIDLPGP SYTIFRQGDS SFFIYSCPSG SKVKDRMIYA SNKNGFINYL KNDQKIAFSK VVEIGDFVEL DKSLLMATNK EDSLDHGSNP DLPNKSNLKF NKPKGPLRKR RT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. Prevents actin filament assembly by forming a 1:1 complex with actin monomers, and inhibits the nucleotide exchange reaction of actin monomers.
  • 基因功能参考文献：
    1. Data show that Twinfilin and Srv2 proteins accelerate depolymerization from actin filament ends. PMID: 26458246
    2. binding of twinfilin to capping protein does not affect the binding of either protein to actin PMID: 15282541
    3. Together, our data suggest that twinfilin coordinates filament severing and monomer sequestering at sites of rapid actin turnover and is controlled by multiple regulatory inputs. PMID: 16569665
  • 亚细胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Actin-binding proteins ADF family, Twinfilin subfamily
  • 数据库链接：

    KEGG: sce:YGR080W

    STRING: 4932.YGR080W