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  4. Recombinant Saccharomyces cerevisiae Ty transcription activator TEC1 (TEC1)

Recombinant Saccharomyces cerevisiae Ty transcription activator TEC1 (TEC1)

  • 货号：
    CSB-YP323847SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP323847SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP323847SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP323847SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP323847SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TEC1
  • Uniprot No.：
  • 别名：
    TEC1; ROC1; YBR083W; YBR0750; Ty transcription activator TEC1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-486
  • 氨基酸序列
    SLKEDDFGK DNSRNIESYT GRIFDVYIQK DSYSQSALDD MFPEAVVSTA ACVKNEAEDN INLIDTHPQF ELVNTGLGAK SDDLKSPSAK ATFTDKQRKN EVPNISVSNY FPGQSSETSS TTESWTIGCD KWSEKVEEAF LEALRLIMKN GTTKIKIRNA NFGRNELISL YIKHKTNEFR TKKQISSHIQ VWKKTIQNKI KDSLTLSSKE KELLHLIEHG AEQTTENSNL FYDIFEEIID SLPSVSDSGS LTPKNLYVSN NSSGLSVHSK LLTPITASNE KKIENFIKTN AASQAKTPLI YAKHIYENID GYKCVPSKRP LEQLSPTELH QGDRPNKASF SNKKAILESA KKIEIEQRKI INKYQRISRI QEHESNPEFS SNSNSGSEYE SEEEVVPRSA TVTQLQSRPV PYYKNNGMPY SLSKVRGRPM YPRPAEDAYN ANYIQGLPQY QTSYFSQLLL SSPQHYEHSP HQRNFTPSNQ SHGNFY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    TEC1 is involved in the activation of TY1 and TY1-mediated gene expression. It is not involved in mating or sporulation processes.
  • 基因功能参考文献：
    1. Collectively, our results implicate Ste12 and Tec1 as general and important contributors to the Cdk8, RNAPII-CTD regulatory circuitry as it relates to the maintenance of genome integrity PMID: 26496706
    2. Data suggest that Tec1 is able to regulate TCS-mediated gene expression by Ste12-dependent and Ste12-independent mechanisms that enable promoter-specific transcriptional control. PMID: 20118212
    3. Tec1 regulates signaling of mitogen-activated protein kinase pathways in yeast PMID: 19826484
    4. Flo8 and Mss11 bind indirectly to UAS2-1 of the STA1 promoter by interacting with Ste12 and Tec1 PMID: 15485921
    5. Tec1 is rapidly degraded during pheromone response through the SCFCdc4 ubiquitin ligase. PMID: 15620356
    6. pheromone signaling induces Fus3-dependent degradation of Tec1, the transcription factor specific to the filamentation pathway PMID: 15620357
    7. pheromone-regulated sumoylation of Ste12 and Tec1 promotes a developmental switch from the invasive to the mating differentiation program PMID: 16306045

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TEC1 family
  • 数据库链接：

    KEGG: sce:YBR083W

    STRING: 4932.YBR083W