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  4. Recombinant Saccharomyces cerevisiae UV excision repair protein RAD23 (RAD23)

Recombinant Saccharomyces cerevisiae UV excision repair protein RAD23 (RAD23)

  • 中文名称：
    酿酒酵母RAD23重组蛋白
  • 货号：
    CSB-YP341343SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母RAD23重组蛋白
  • 货号：
    CSB-EP341343SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母RAD23重组蛋白
  • 货号：
    CSB-EP341343SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母RAD23重组蛋白
  • 货号：
    CSB-BP341343SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母RAD23重组蛋白
  • 货号：
    CSB-MP341343SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RAD23
  • Uniprot No.：
  • 别名：
    RAD23; YEL037C; SYGP-ORF29; UV excision repair protein RAD23
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-398
  • 氨基酸序列
    MVSLTFKNFK KEKVPLDLEP SNTILETKTK LAQSISCEES QIKLIYSGKV LQDSKTVSEC GLKDGDQVVF MVSQKKSTKT KVTEPPIAPE SATTPGRENS TEASPSTDAS AAPAATAPEG SQPQEEQTAT TERTESASTP GFVVGTERNE TIERIMEMGY QREEVERALR AAFNNPDRAV EYLLMGIPEN LRQPEPQQQT AAAAEQPSTA ATTAEQPAED DLFAQAAQGG NASSGALGTT GGATDAAQGG PPGSIGLTVE DLLSLRQVVS GNPEALAPLL ENISARYPQL REHIMANPEV FVSMLLEAVG DNMQDVMEGA DDMVEGEDIE VTGEAAAAGL GQGEGEGSFQ VDYTPEDDQA ISRLCELGFE RDLVIQVYFA CDKNEEAAAN ILFSDHAD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a central role both in proteasomal degradation of misfolded proteins and DNA repair. Central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. Involved in DNA excision repair. May play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes.
  • 基因功能参考文献：
    1. Rad4-Rad23 forms non-motile complexes or conducts a one-dimensional search for DNA lesions via either random diffusion or constrained motion. PMID: 27720644
    2. these studies are consistent with a model in which Rad23p partners with the deglycosylating enzyme Png1p to reduce manganese toxicity through proteosomal degradation of glycosylated substrate(s). PMID: 22281500
    3. C-terminal UBA domain of Rad23 ubiquitin receptor protects it by preventing initiation of protein degradation at the proteasome. PMID: 21304520
    4. Rad23 escapes degradation because it lacks a proteasome initiation region. PMID: 21304521
    5. Role of Rad23 protein in DNA repair. PMID: 15545636
    6. Rad23 stabilizes Rad4 from degradation during nucleotide excision-repair. PMID: 15601997
    7. Results reveal that Rad23 and Ddi1 interact with each other by using UBL/UBA domain interactions in a manner that does not preclude their interaction with ubiquitin. PMID: 16405905
    8. determined that hHR23A and hHR23B could be co-purified with unique proteolytic and stress-responsive factors from human breast cancer tissues, indicating that they have unique functions in vivo PMID: 16712842
    9. Snf6 and Snf5, two subunits of the SWI/SNF chromatin-remodeling complex in Saccharomyces cerevisiae, copurify with the NER damage-recognition heterodimer Rad4-Rad23. This interaction between SWI/SNF and Rad4-Rad23 is stimulated by UV irradiation. PMID: 17013386
    10. recognition of polyubiquitin chains by Rad23 promotes its shuttling to the proteasome in vivo PMID: 17360968
    11. Mutations in the hydrophobic core of ubiquitin affect its recognition by RAD23. PMID: 18054791
    12. Rad23, originally identified as an important factor involved in the recognition of DNA lesions, also plays a central role in targeting ubiquitylated proteins for proteasomal degradation [Review] PMID: 19223247

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 数据库链接：

    KEGG: sce:YEL037C

    STRING: 4932.YEL037C