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  4. Recombinant Saccharomyces cerevisiae Ubiquitin-like protein MDY2 (MDY2)

Recombinant Saccharomyces cerevisiae Ubiquitin-like protein MDY2 (MDY2)

  • 中文名称：
    酿酒酵母MDY2重组蛋白
  • 货号：
    CSB-YP614200SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母MDY2重组蛋白
  • 货号：
    CSB-EP614200SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母MDY2重组蛋白
  • 货号：
    CSB-EP614200SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母MDY2重组蛋白
  • 货号：
    CSB-BP614200SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母MDY2重组蛋白
  • 货号：
    CSB-MP614200SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MDY2
  • Uniprot No.：
  • 别名：
    MDY2; GET5; TMA24; YOL111CUbiquitin-like protein MDY2; Golgi to ER traffic protein 5; Mating-deficient protein 2; Translation machinery-associated protein 24
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-212
  • 氨基酸序列
    MSTSASGPEH EFVSKFLTLA TLTEPKLPKS YTKPLKDVTN LGVPLPTLKY KYKQNRAKKL KLHQDQQGQD NAAVHLTLKK IQAPKFSIEH DFSPSDTILQ IKQHLISEEK ASHISEIKLL LKGKVLHDNL FLSDLKVTPA NSTITVMIKP NPTISKEPEA EKSTNSPAPA PPQELTVPWD DIEALLKNNF ENDQAAVRQV MERLQKGWSL AK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.
  • 基因功能参考文献：
    1. The nuclear history of the UBL Mdy2 appears to be essential for its function in cytoplasmic stress granules during the rapid cellular response to heat stress. PMID: 23285234
    2. Structures of the Sgt2/SGTA dimerization domain with the Get5/UBL4A UBL domain reveal an interaction that forms a conserved dynamic interface. PMID: 23142665
    3. solve a structure of the Sgt2_NT/Get5_UBL complex, validate it via site-directed mutagenesis, and empirically determine its stoichiometry using relaxation experiments and isothermal titration calorimetry PMID: 23297211
    4. Get5 carboxyl-terminal domain is a novel dimerization motif that tethers an extended Get4/Get5 complex. PMID: 22262836
    5. a structural model of the Sgt2-Get4/Get5-HSC complex. PMID: 21832041
    6. TA protein sorting defects are more severe in sgt2Delta get5Delta mutants compared to single knockouts PMID: 21619481
    7. Data show that MDY2 is a novel gene, whose product plays a role in shmoo formation and in nuclear migration in the pre-zygote. PMID: 16390866
    8. These results suggest that protein complexes containing Sgt2 and Mdy2 bring molecular chaperones together to carry out certain chaperoning functions. PMID: 17441508

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  • 亚细胞定位：
    Cytoplasm, cytosol. Nucleus. Note=Detected in both cytosol and nucleus but is predominantly cytosolic.
  • 数据库链接：

    KEGG: sce:YOL111C

    STRING: 4932.YOL111C