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  4. Recombinant Saccharomyces cerevisiae Uncharacterized protein YJR129C (YJR129C)

Recombinant Saccharomyces cerevisiae Uncharacterized protein YJR129C (YJR129C)

  • 货号：
    CSB-YP342865SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP342865SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP342865SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP342865SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP342865SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EFM3
  • Uniprot No.：
  • 别名：
    EFM3; YJR129C; J2061Protein-lysine N-methyltransferase EFM3; EC 2.1.1.-; Elongation factor methyltransferase 3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-339
  • 氨基酸序列
    MNEDLFYDRL HQRCPGKYLL EELETSKSND VLHASRFVCE MELVQKTNAY YCKTIVKMLL DHEWIFAKAF TIVNDGEDEI EIYDYLYEKY IKLLSTGKPD PMMKDVVRYR FDEDVKIKIE ETPNLISAAS TTGFRTWEAA LYMGDFLIHK PLQELAPVQG QDDGKKKLNV LEVGAGTGIV SLVILQKYHE FVNKMYVTDG DSNLVETQLK RNFELNNEVR ENEPDIKLQR LWWGSDRVPE DIDLVVGADV TYDPTILPDL CECLAECLAL DRCKLCLLSA TIRSESTVQL FSQECNKLGL KCTIVTSTEY DANNEIRAMK ALQFKPLIAP IRIYKITKQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that mono-, di- and trimethylates elongation factor 2 (EFT1/EFT2) at 'Lys-509'.
  • 基因功能参考文献：
    1. Previously uncharacterized lysine-specific methyltransferase FAM86A and Yjr129c introduce a functionally important lysine methylation in eEF2. PMID: 25231979
    2. Propose that YJR129C be named elongation factor methyltransferase 3 (Efm3). An immunoblotting screen of 21 putative methyltransferases showed a loss in the methylation of elongation factor 2 (EF2) on knockout of YJR129C. PMID: 25086354
    3. the contribution of the ER24 gene product Ylr376Cp and of the two suppressor gene products Yjl185Cp and Yjr129Cp to a complex regulation of the glyoxylate cycle in yeast PMID: 17146497
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, EEF2KMT family
  • 数据库链接：

    KEGG: sce:YJR129C

    STRING: 4932.YJR129C