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  4. Recombinant Saccharomyces cerevisiae cAMP-dependent protein kinase type 1 (TPK1)

Recombinant Saccharomyces cerevisiae cAMP-dependent protein kinase type 1 (TPK1)

  • 中文名称：
    酿酒酵母TPK1重组蛋白
  • 货号：
    CSB-YP361759SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母TPK1重组蛋白
  • 货号：
    CSB-EP361759SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母TPK1重组蛋白
  • 货号：
    CSB-EP361759SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母TPK1重组蛋白
  • 货号：
    CSB-BP361759SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母TPK1重组蛋白
  • 货号：
    CSB-MP361759SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TPK1
  • Uniprot No.：
  • 别名：
    TPK1; PKA1; SRA3; YJL164C; J0541; cAMP-dependent protein kinase type 1; PKA 1; EC 2.7.11.11; CDC25-suppressing protein kinase; PK-25
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-397
  • 氨基酸序列
    STEEQNGGG QKSLDDRQGE ESQKGETSER ETTATESGNE SKSVEKEGGE TQEKPKQPHV TYYNEEQYKQ FIAQARVTSG KYSLQDFQIL RTLGTGSFGR VHLIRSRHNG RYYAMKVLKK EIVVRLKQVE HTNDERLMLS IVTHPFIIRM WGTFQDAQQI FMIMDYIEGG ELFSLLRKSQ RFPNPVAKFY AAEVCLALEY LHSKDIIYRD LKPENILLDK NGHIKITDFG FAKYVPDVTY TLCGTPDYIA PEVVSTKPYN KSIDWWSFGI LIYEMLAGYT PFYDSNTMKT YEKILNAELR FPPFFNEDVK DLLSRLITRD LSQRLGNLQN GTEDVKNHPW FKEVVWEKLL SRNIETPYEP PIQQGQGDTS QFDKYPEEDI NYGVQGEDPY ADLFRDF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 基因功能参考文献：
    1. These results show that rapid activation of the TOR pathway by amino acids is not involved in rapid activation of the PKA pathway and that effects of Gcn2 inactivation as well as leu2 auxotrophy all act independently of the TOR pathway. PMID: 28810702
    2. Long non-coding RNA IRT1 transcription is repressed when nutrients are ample and target of rapamycin complex I (TORC1) and protein kinase A (PKA) are active. PMID: 27520925
    3. protein kinase A (PKA) and target of rapamycin complex I (TORC1) signalling mediate nutrient regulation of IME1 expression. PMID: 27272508
    4. Data indicate that Rgt1 and tpk1/2/3 (cAMP-dependent protein kinases) participate in the control of SUC2 expression. PMID: 25810078
    5. results describe that an autophosphorylation mechanism on Tpk1 controls PKA activity in response to glucose availability PMID: 24947305
    6. Trehalase activation is associated with phosphorylation of typical PKA sites. PMID: 23155055
    7. Tpk1 and Tpk2 interact with Pkh1 through a hydrophobic motif-PDK1-interacting fragment pocket interaction. PMID: 22957732
    8. The s show that the evolutionarily conserved protein kinases Atg1, target of rapamycin kinase complex I, and protein kinase A (PKA) regulate autophagic flux, whereas autophagy gene induction depends solely on PKA. PMID: 21468027
    9. S. cerevisiae DPMS activity is regulated by the cAMP-dependent protein phosphorylation signal, and the phosphorylation target is serine 141 PMID: 15548536
    10. Tor and cyclic AMP-protein kinase A regulate expression of genes required for cell growth PMID: 15643061
    11. Tpk1p phosphorylation is dynamic and reversible: its up-regulation correlates with a fully fermentative metabolism, while its down-regulation with stationary phase or respiratory metabolism. PMID: 16226873

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, cAMP subfamily
  • 数据库链接：

    KEGG: sce:YJL164C

    STRING: 4932.YJL164C