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  4. Recombinant Saccharomyces cerevisiae eRF1 methyltransferase catalytic subunit MTQ2 (MTQ2)

Recombinant Saccharomyces cerevisiae eRF1 methyltransferase catalytic subunit MTQ2 (MTQ2)

  • 中文名称：
    酿酒酵母MTQ2重组蛋白
  • 货号：
    CSB-YP312750SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母MTQ2重组蛋白
  • 货号：
    CSB-EP312750SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母MTQ2重组蛋白
  • 货号：
    CSB-EP312750SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母MTQ2重组蛋白
  • 货号：
    CSB-BP312750SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母MTQ2重组蛋白
  • 货号：
    CSB-MP312750SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MTQ2
  • Uniprot No.：
  • 别名：
    MTQ2; MTC6; YDR140W; eRF1 methyltransferase catalytic subunit MTQ2; eRF1 MTase catalytic subunit MTQ2; EC 2.1.1.297; N(5)-glutamine methyltransferase MTQ2
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-221
  • 氨基酸序列
    MLPTPYVKCD YDKVYEPAED SFLILDCLEK EHDFLKQKFG NRLAIVCEIG SGSGIVTTFL MQNKIIPQEN SIHLAVDINP WALEATLDTA KLNSCKSSFL EVIQADLNSS IRNNQVDVLI FNPPYVPAEC VPDVPGSREE ADQWLDLALL GGKDGMAITD KLLRQLEQIL SPDGVAYILF CARNKPKEVI KRFVDTYKWN VKLIETRKAG WEVLSVYSFT R
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Methylates eRF1 on 'Gln-182' using S-adenosyl L-methionine as methyl donor. eRF1 needs to be complexed to eRF3 in its GTP-bound form to be efficiently methylated.
  • 基因功能参考文献：
    1. the natural substrate of the methyltransferase YDR140w is the ternary complex eRF1.eRF3.GTP PMID: 15509572
    2. Mtq2p methylates Sup45p the mtq2-Delta had multiple phenotypes, including cold sensitivity and sensitivity to translation fidelity antibiotics paromomycin and geneticin, to high salt and calcium concentrations, to polymyxin B, and to caffeine PMID: 16321977
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Eukaryotic/archaeal PrmC-related family
  • 数据库链接：

    KEGG: sce:YDR140W

    STRING: 4932.YDR140W