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  4. Recombinant Saccharomyces cerevisiae tRNA-splicing endonuclease subunit SEN34 (SEN34)

Recombinant Saccharomyces cerevisiae tRNA-splicing endonuclease subunit SEN34 (SEN34)

  • 中文名称：
    酿酒酵母SEN34重组蛋白
  • 货号：
    CSB-YP025121SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母SEN34重组蛋白
  • 货号：
    CSB-EP025121SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母SEN34重组蛋白
  • 货号：
    CSB-EP025121SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母SEN34重组蛋白
  • 货号：
    CSB-BP025121SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母SEN34重组蛋白
  • 货号：
    CSB-MP025121SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SEN34
  • Uniprot No.：
  • 别名：
    SEN34; YAR008W; FUN4; tRNA-splicing endonuclease subunit SEN34; EC 4.6.1.16; Splicing endonuclease of 34 kDa; tRNA-intron endonuclease SEN34
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-275
  • 氨基酸序列
    MPPLVFDIDH IKLLRKWGIC GVLSGTLPTA AQQNVFLSVP LRLMLEDVLW LHLNNLADVK LIRQEGDEIM EGITLERGAK LSKIVNDRLN KSFEYQRKFK KDEHIAKLKK IGRINDKTTA EELQRLDKSS NNDQLIESSL FIDIANTSMI LRDIRSDSDS LSRDDISDLL FKQYRQAGKM QTYFLYKALR DQGYVLSPGG RFGGKFIAYP GDPLRFHSHL TIQDAIDYHN EPIDLISMIS GARLGTTVKK LWVIGGVAEE TKETHFFSIE WAGFG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage.
  • 基因功能参考文献：
    1. Sen34p is absolutely required for tRNA splicing and may affect also rRNA maturation. PMID: 16188229
  • 亚细胞定位：
    Nucleus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note=The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface.
  • 蛋白家族：
    TRNA-intron endonuclease family
  • 数据库链接：

    KEGG: sce:YAR008W

    STRING: 4932.YAR008W