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  4. Recombinant Salmonella typhimurium Putative transcriptional regulatory protein ptsJ (ptsJ)

Recombinant Salmonella typhimurium Putative transcriptional regulatory protein ptsJ (ptsJ)

  • 货号：
    CSB-YP336691SXB
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP336691SXB
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP336691SXB-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP336691SXB
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP336691SXB
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ptsJ
  • Uniprot No.：
  • 别名：
    ptsJ; STM2436; Vitamin B6 salvage pathway transcriptional repressor PtsJ; HTH-type transcriptional regulatory protein PtsJ
  • 种属：
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-430
  • 氨基酸序列
    MIDGKTANEI FDSIRQHIIA GTLRAEDSLP PVRELASELK VNRNTVAAAY KRLITAGLAQ SLGRNGTVIK GSPSPVALEG GDPHTPLHDL SGGNPDPQRL PDLSRYFARL SRTPHLYGDA PVSPELHAWA ARWLRDATPV AGEIDITSGA IDAIERLLCA HLLPGDSVAV EDPCFLSSIN MLRYAGFSAS PVSVDSEGMQ PEKLERALNQ GARAVILTPR AHNPTGCSLS ARRAAALQNM LARYPQVVVI IDDHFALLSS SPWQPVIAQT TQHWAVIRSV SKTLGPDLRL AIVASDSATS AKLRLRLNAG SQWVSHLLQD LVYACLTDPE YQHRLTQTRL FYAARQQKLA RALQQYGIAI SPGDGVNAWL PLDTHSQATA FTLAKSGWLV REGEAFGVSA PSHGLRITLS TLNDSEINTL AADIHQALNR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as transcriptional repressor of the pdxK gene, encoding a pyridoxal kinase involved in the vitamin B6 salvage pathway. Also represses transcription of its own gene. Binds to the ptsJ-pdxK intergenic region, but does not bind pdxY and pdxH promoters. Among all six B6 vitamers, only pyridoxal 5'-phosphate (PLP) clearly binds to the protein and acts as an effector molecule for PtsJ, inducing a protein conformational change that increases affinity for DNA. Thus, PLP stabilizes protein-DNA interactions, reinforcing repression.
  • 基因功能参考文献：
    1. PtsJ is a novel MocR-like transcriptional repressor involved in regulating the vitamin B6 salvage pathway. PMID: 27987384
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family
  • 数据库链接：

    KEGG: stm:STM2436

    STRING: 99287.STM2436