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  4. Recombinant Schizosaccharomyces pombe Postreplication repair E3 ubiquitin-protein ligase rad18 (rhp18)

Recombinant Schizosaccharomyces pombe Postreplication repair E3 ubiquitin-protein ligase rad18 (rhp18)

  • 中文名称：
    庞贝裂殖酵母rhp18重组蛋白
  • 货号：
    CSB-YP527950SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    庞贝裂殖酵母rhp18重组蛋白
  • 货号：
    CSB-EP527950SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    庞贝裂殖酵母rhp18重组蛋白
  • 货号：
    CSB-EP527950SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    庞贝裂殖酵母rhp18重组蛋白
  • 货号：
    CSB-BP527950SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    庞贝裂殖酵母rhp18重组蛋白
  • 货号：
    CSB-MP527950SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rhp18
  • Uniprot No.：
  • 别名：
    rhp18; SPBC1734.06; Postreplication repair E3 ubiquitin-protein ligase rad18; EC 2.3.2.27; RAD18 homolog; RING-type E3 ubiquitin transferase rad18
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-387
  • 氨基酸序列
    MNELDATDPS DWNQTKIPSL KGLDSSLRCL ICHEYFRAPL ITSCSHTFCS FCIRDYLREH PMCPACRAPE QESRLRKNTI LEEILESFKV IRPTLFEFLK VENVPKPVLQ APETVIAQDS ASGDEEWEDD LASNSSPASI AKKTSRDSKK RKREDLVHCP ACSNLVPHNQ INQHLDSCLN SPSSPSSSSS PYKNKDNSKS NSLLSFKTDD DSITKRRLRS FNSADELPLK DRVRLPKLTY ALLSESKIRS KLSEMGLPTD GHKQLLQRRH AKWVTLYNSN LDQKQPVSKR NLIRQLIDWE RVQSKSIGVE KEKLGGGDWE KAYAEDFADL INRAKQSTTN KNDSLRNTAV ESSTEPSTSN GFPATSVSPP LTIDLTNSQT GSDGPQS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 RING-finger protein, member of the UBC2/RAD6 epistasis group. Associates to the E2 ubiquitin conjugating enzyme ubc2/rad6 to form the ubc2-rad18 ubiquitin ligase complex involved in postreplicative repair (PRR) of damaged DNA.
  • 基因功能参考文献：
    1. Using these screens and immunoblot measures of Tf1 proteins, we identified a total of 61 genes that promote integration. Two candidates, Rhp18 and the NineTeen complex were tested in two-hybrid assays and were found to interact with Tf1 IN. PMID: 29232693
    2. Report identified Rhp18 as another binding partner of Brc1 which could be important for replication forks. PMID: 28784724
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RAD18 family
  • 数据库链接：

    KEGG: spo:SPBC1734.06

    STRING: 4896.SPBC1734.06.1