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  4. Recombinant Schizosaccharomyces pombe Protein spt3 (spt3)

Recombinant Schizosaccharomyces pombe Protein spt3 (spt3)

  • 中文名称：
    庞贝裂殖酵母spt3重组蛋白
  • 货号：
    CSB-YP522671SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    庞贝裂殖酵母spt3重组蛋白
  • 货号：
    CSB-EP522671SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    庞贝裂殖酵母spt3重组蛋白
  • 货号：
    CSB-EP522671SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    庞贝裂殖酵母spt3重组蛋白
  • 货号：
    CSB-BP522671SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    庞贝裂殖酵母spt3重组蛋白
  • 货号：
    CSB-MP522671SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    spt3
  • Uniprot No.：
  • 别名：
    spt3; SPCC61.02; SAGA complex subunit spt3
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-307
  • 氨基酸序列
    MTETGRTSKY RVEIQQMMFI LGEVQDPLPE TTQLVEELIR GQVMEMLIQA NELALRRGSR SITVEDLFFL IRHDRAKVNR LKTYLSWKEV RKKAKEQDAN PADTKDLFEE VDSKTRSMNA VAMPWEVKNM FTEPVPETEE FEEDNDTMEM AYATRQRLKM ADERTKKMTR EEYVHWSECR QASFTYRKGK RFREWCGMSI LTDTRPDNDI VDILGFLTFE IVATLTEEAL AVKDRMDRIQ NSSGSGGSHA AHHPKNCSLF DGLTEGRTPL QVSHVLEAFR RLQIPDKTHT AMRSFHGGLV KSRVYLI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as component of the transcription regulatory histone acetylation (HAT) complex SAGA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (spt3, spt8 and spt20) and promoter selectivity, interaction with transcription activators (gcn5, ada2, ada3 and tra1), and chromatin modification through histone acetylation (gcn5) and deubiquitination (ubp8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). Spt3 is required for recruitment of TATA-binding protein (TBP) to SAGA-dependent promoters. During SAGA-mediated transcriptional inhibition, spt3 and spt8 prevent binding of TBP to the TATA box. SPT factors 3, 7 and 8 are required for the initiation of Ty transcription from the delta promoter. Spt3 regulates Ty1 as well as the mating factor genes.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    SPT3 family
  • 数据库链接：

    KEGG: spo:SPCC61.02

    STRING: 4896.SPCC61.02.1