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  4. Recombinant Staphylococcus epidermidis Lantibiotic epidermin (epiA)

Recombinant Staphylococcus epidermidis Lantibiotic epidermin (epiA)

  • 中文名称：
    表皮葡萄球菌epiA重组蛋白
  • 货号：
    CSB-EP357305FLL
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    epiA
  • Uniprot No.：
  • 别名：
    epiALantibiotic epidermin
  • 种属：
    Staphylococcus epidermidis
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    17.7 kDa
  • 表达区域：
    31-52aa
  • 氨基酸序列
    IASKFICTPGCAKTGSFNSYCC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-KSI-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    In the production of recombinant S. epidermidis epiA protein, the gene for epiA (E.coli) was cloned into a vector and expressed as epiA protein in E.coli. The plasmids with the copy of epiA, or the expression vector, were often used to enhance gene expression. Every step of production was undergone with a strict QC system. N-terminal 6xHis-KSI tag was used in the process. The purity is 85% determined by SDS-PAGE.

    The Staphylococcus epidermidis genes encoding the His-tag-labelled epidermin precursor peptide EpiA. The 52-amino acid prepeptide EpiA consists of an N-terminal leader peptide with amino acids - 30 to - 1 and a C-terminal pro-epidermin with amino acids + 1 to + 22. EpiA is post-translationally converted to the mature antibiotic by dehydration of serine and threonine residues, sulfide bridge formation, oxidative decarboxylation at the C-terminus and removal of the leader peptide. enzymes involved in epidermin biosynthesis. Studies identified that EpiA was expressed in Escherichia coli using a malE-epiA fusion. The identity of purified EpiA was confirmed by ion spray mass spectrometry and amino acid sequencing. For EpiA detection, anti-EpiA antisera were raised. Upon prolonged incubation, factor Xa not only cleaved EpiA from the fusion protein, but also less efficiently cleaved EpiA internally between R-1 and I + 1.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
  • 蛋白家族：
    Type A lantibiotic family