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  4. Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin (ply)

Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin (ply)

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  • 中文名称：
    Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin(ply)
  • 货号：
    CSB-BP314690FMW
  • 规格：
    ￥3168
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Baculovirus host and target protein, the LC-MS/MS Analysis result of CSB-BP314690FMW could indicate that this peptide derived from Baculovirus-expressed Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) ply.
    • Based on the SEQUEST from database of Baculovirus host and target protein, the LC-MS/MS Analysis result of CSB-BP314690FMW could indicate that this peptide derived from Baculovirus-expressed Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) ply.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    ply
  • Uniprot No.：
  • 别名：
    ply; SP_1923; Pneumolysin; Ply; Thiol-activated cytolysin
  • 种属：
    Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Baculovirus
  • 分子量：
    56.8 kDa
  • 表达区域：
    2-471aa
  • 氨基酸序列
    ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The synthesis of Recombinant S.pneumoniae serotype 4 ply protein began with the isolation of ply gene from the native genome. The next step was to amplify the ply gene by inserting the ply gene into an expression vector and then introducing the vector into the selected host cell, Baculovirus. Significant amounts of recombinant S.pneumoniae serotype 4 ply protein were produced by the host only when expression genes were added. For the purpose of protein purification, the vector contains N-terminal 10xHis tag & C-terminal Myc tag to the ply DNA sequence. The purity of this protein is 90%+ determined by SDS-PAGE.

    ply (also known as SP_1923) is a gene encoding a protein named pneumolysin (ply) in Streptococcus pneumoniae serotype 4. The protein encoded by this gene is also known as thiol-activated cytolysin and belongs to Thiol-activated cytolysin family. The molecular weight of this protein is 53 kDa composed of 471 amino acids. It has a multiple biological activity, including the ability to lyse and interfere with the function of cells and soluble molecules of the immune system.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
  • 亚细胞定位：
    Secreted. Secreted, cell wall. Host cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Thiol-activated cytolysin family
  • 数据库链接：

    KEGG: spn:SP_1923