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  4. Recombinant Thermotoga maritima Endonuclease V (nfi)

Recombinant Thermotoga maritima Endonuclease V (nfi)

  • 中文名称：
    马里提马热托加nfi重组蛋白
  • 货号：
    CSB-YP894909TNJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    马里提马热托加nfi重组蛋白
  • 货号：
    CSB-EP894909TNJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    马里提马热托加nfi重组蛋白
  • 货号：
    CSB-EP894909TNJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    马里提马热托加nfi重组蛋白
  • 货号：
    CSB-BP894909TNJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    马里提马热托加nfi重组蛋白
  • 货号：
    CSB-MP894909TNJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    nfi
  • Uniprot No.：
  • 别名：
    nfi; TM_1865; Endonuclease V; EC 3.1.21.7; Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V
  • 种属：
    Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-225
  • 氨基酸序列
    MDYRQLHRWD LPPEEAIKVQ NELRKKIKLT PYEGEPEYVA GVDLSFPGKE EGLAVIVVLE YPSFKILEVV SERGEITFPY IPGLLAFREG PLFLKAWEKL RTKPDVVVFD GQGLAHPRKL GIASHMGLFI EIPTIGVAKS RLYGTFKMPE DKRCSWSYLY DGEEIIGCVI RTKEGSAPIF VSPGHLMDVE SSKRLIKAFT LPGRRIPEPT RLAHIYTQRL KKGLF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. In vitro, can also cleave single-stranded substrates with inosine, double-stranded DNA with apurinic sites, or DNA sites with uracil or a mismatched base. When present in molar excess, two protein molecules can bind to the same DNA substrate and effect cleavage of both strands (in vitro).
  • 基因功能参考文献：
    1. A novel model for DNA loop recognition and cleavage by EndoV, in which a coordinated action of a DNA-intercalating wedge and a base pocket accommodating a flipped normal base facilitate strand incision are revealed by X-ray crystallography. PMID: 23313664
    2. determined the single-molecule kinetics of endo V in a catalytic cycle using a substrate of deoxyinosine-containing single-stranded DNA PMID: 17521169
    3. Study reports crystal structures of EndoV in complex with a hypoxanthine lesion substrate and product DNA; the PYIP wedge motif acts as a minor groove-damage sensor for helical distortions and base mismatches and separates DNA strands at the lesion. PMID: 19136958
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Endonuclease V family
  • 数据库链接：

    KEGG: tma:TM1865

    STRING: 243274.TM1865