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  4. Recombinant Tobacco mosaic virus Movement protein (MP)

Recombinant Tobacco mosaic virus Movement protein (MP)

  • 货号：
    CSB-YP356090TFF
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP356090TFF
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP356090TFF-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP356090TFF
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP356090TFF
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MP
  • Uniprot No.：
  • 别名：
    MP; MP30; Movement protein; 30 kDa protein; Cell-to-cell transport protein) [Cleaved into: 27.9 kDa protein; 25.3 kDa protein; 19.5 kDa protein]
  • 种属：
    Tobacco mosaic virus (strain vulgare) (TMV) (Tobacco mosaic virus (strain U1))
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-268
  • 氨基酸序列
    MALVVKGKVN INEFIDLTKM EKILPSMFTP VKSVMCSKVD KIMVHENESL SEVNLLKGVK LIDSGYVCLA GLVVTGEWNL PDNCRGGVSV CLVDKRMERA DEATLGSYYT AAAKKRFQFK VVPNYAITTQ DAMKNVWQVL VNIRNVKMSA GFCPLSLEFV SVCIVYRNNI KLGLREKITN VRDGGPMELT EEVVDEFMED VPMSIRLAKF RSRTGKKSDV RKGKNSSNDR SVPNKNYRNV KDFGGMSFKK NNLIDDDSEA TVAESDSF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Forms a ribonucleoprotein complex with viral RNA. Binds microtubules and modulates microtubule stability. Can bind double-stranded DNA. Triggers host hypersensitive defense reaction in incompatible plants harboring resistance (R) proteins.
  • 基因功能参考文献：
    1. Data show that ToMV resistance protein Tm-2(2) interacts with Tobacco mosaic virus (TMV) viral movement protein (MP) to form a protein complex at the plasma membrane (PM). PMID: 28258211
    2. The s identify of a bona fide functional Tobacco mosaic virus MP plasmodesmal localization signal, which encompasses amino acid residues between positions 1 and 50, with residues Val-4 and Phe-14 representing critical sites for function. PMID: 26787834
    3. Arabidopsis (Arabidopsis thaliana) CDC48 is induced upon infection, interacts with MP in endoplasmic reticulum inclusions dependent on the MP N terminus, and promotes degradation of the protein. PMID: 23027663
    4. This Study considered the role of mutations in the transport protein and its binding to viral rna to form a complex. PMID: 15523831
    5. Results indicate that the size difference of necrotic lesions induced by tobacco and tomato mosaic viruses in tobacco plants containing the N gene results from the functional difference of their movement protein genes. PMID: 15620106
    6. During viral infection in Tobacco, protein is phosphorylated. PMID: 15680421
    7. the function of movement protein in TMV viral movement may involve interaction with the microtubule-nucleating machinery. PMID: 16731920
    8. functions as a genuine microtubule-associated protein that binds microtubules directly and modulates microtubule stability. PMID: 16912284
    9. TMV exhibits the activity of a suppressor of viral RNA silencing. PMID: 16986444
    10. Data supports a diffusion model in which a complex that includes endoplasmic reticulum (ER)-embedded movement protein, viral RNA, and other components diffuses in the ER membrane within the plasmodesmata. PMID: 18257683
    11. microtubule network performs anchorage and release functions for controlling the assembly and intracellular movement of movement protein-containing RNA transport particles in association with the endoplasmic reticulum PMID: 19281527
    12. NtMPIP1, a novel subset of DnaJ-like proteins, was identified from a tobacco cDNA library, and its specific interaction with TMV MP was confirmed with an in vitro filter-binding assay. PMID: 19458900

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  • 亚细胞定位：
    Host cytoplasm, host cytoskeleton. Host cell junction, host plasmodesma.
  • 蛋白家族：
    Tobamovirus movement protein family
  • 数据库链接：

    KEGG: vg:1494078