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  4. Recombinant Toxin PezT (pezT)

Recombinant Toxin PezT (pezT)

  • 货号：
    CSB-YP857202FMW
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP857202FMW
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP857202FMW-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP857202FMW
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP857202FMW
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    pezT
  • Uniprot No.：
  • 别名：
    pezT; SP_1051; Toxin PezT; UDP-N-acetylglucosamine kinase; UNAG kinase; EC 2.7.1.176; Zeta toxin
  • 种属：
    Streptococcus pneumoniae
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-253
  • 氨基酸序列
    MEIQDYTDSE FKHALARNLR SLTRGKKSSK QPIAILLGGQ SGAGKTTIHR IKQKEFQGNI VIIDGDSFRS QHPHYLELQQ EYGKDSVEYT KDFAGKMVES LVTKLSSLRY NLLIEGTLRT VDVPKKTAQL LKNKGYEVQL ALIATKPELS YLSTLIRYEE LYIINPNQAR ATPKEHHDFI VNHLVDNTRK LEELAIFERI QIYQRDRSCV YDSKENTTSA ADVLQELLFG EWSQVEKEML QVGEKRLNEL LEK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Toxic component of a type II toxin-antitoxin (TA) system. Phosphorylates UDP-N-acetyl-D-glucosamine (UNAG) on the 3'-hydroxyl group of the N-acetyl-D-glucosamine moiety, yielding UNAG-3P. UNAG-3P inhibits MurA, the first committed step in cell wall synthesis, which is then blocked. Upon expression in E.coli results in decreased cell growth and viability, followed 3 hours later by growth restoration; the toxic effect and phosphorylation of UNAG are neutralized by coexpression with cognate antitoxin PezA. A mutant lacking the last 11 residues is stably maintained in E.coli, unlike the wild-type which undergoes spontaneous mutation. Expression of the deletion mutant in rapidly growing liquid cultures leads to cell bulging, permeabilization and massive lysis by 1 hour. Cells that survive are not able to undergo cytokinesis. Expression in slowly growing cells leads to bulging but not lysis.; Acts as a corepressor of its own operon with PezA; it is not clear if it binds DNA alone.
  • 蛋白家族：
    Zeta toxin family
  • 数据库链接：

    KEGG: spn:SP_1051