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  4. Recombinant Turnip crinkle virus Capsid protein (ORF4)

Recombinant Turnip crinkle virus Capsid protein (ORF4)

  • 中文名称：
    Recombinant Turnip crinkle virus Capsid protein (ORF4),Yeast
  • 货号：
    CSB-YP361910TJL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Turnip crinkle virus Capsid protein (ORF4),Yeast
  • 货号：
    CSB-EP361910TJL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Turnip crinkle virus Capsid protein (ORF4),Yeast
  • 货号：
    CSB-EP361910TJL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Turnip crinkle virus Capsid protein (ORF4),Yeast
  • 货号：
    CSB-BP361910TJL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Turnip crinkle virus Capsid protein (ORF4),Yeast
  • 货号：
    CSB-MP361910TJL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ORF4
  • Uniprot No.：
  • 别名：
    ORF4Capsid protein; Coat protein; p38
  • 种属：
    Turnip crinkle virus (TCV)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-351
  • 氨基酸序列
    MENDPRVRKF ASDGAQWAIK WQKKGWSTLT SRQKQTARAA MGIKLSPVAQ PVQKVTRLSA PVALAYREVS TQPRVSTARD GITRSGSELI TTLKKNTDTE PKYTTAVLNP SEPGTFNQLI KEAAQYEKYR FTSLRFRYSP MSPSTTGGKV ALAFDRDAAK PPPNDLASLY NIEGCVSSVP WTGFILTVPT DSTDRFVADG ISDPKLVDFG KLIMATYGQG ANDAAQLGEV RVEYTVQLKN RTGSTSDAQI GDFAGVKDGP RLVSWSKTKG TAGWEHDCHF LGTGNFSLTL FYEKAPVSGL ENADASDFSV LGEAAAGSVQ WAGVKVAERG QGVKMVTTEE QPKGKLQALR I
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry, about 32-35 nm in diameter, and consisting of 180 capsid proteins. Also acts as a suppressor of RNA-mediated gene silencing known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May suppress other innate immunity by binding to host TIP protein, thereby preventing it to migrate in the nucleus where it could trigger an antiviral response.
  • 基因功能参考文献：
    1. Together, these results uncovered a strong correlation between nuclear localization and nuclear inclusion formation by turnip crinkle virus capsid protein and induction of host hypersensitive response. PMID: 26299399
    2. The N-terminal 52-amino-acid R domain of capsid protein is the elicitor of hypersensitive responses in Nicotiana benthamiana. PMID: 25656064
    3. p38-defective mutant loses the ability to repress cucumber mosaic virus, suggesting that p38 plays an important role in the antagonistic effect toward cucumber mosaic virus in Arabidopsis plants co-infected with both viruses. PMID: 25092463
    4. Turnip crinkle virus coat protein inhibits the basal immune response in Arabidopsis by binding to the TIP protein. PMID: 24418554
    5. Turnip crinkle virus capsid protein not only allows the viral genome to access the systemic movement channel through silencing suppression but also ensures its smooth egress by way of assembled virus particles. PMID: 20504923
    6. The paper demonstrates that a functional coat protein, but not DCL2/DCL4, is required for satC-mediated repression of turnip crinkle virus. PMID: 18639914

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  • 亚细胞定位：
    Virion.
  • 蛋白家族：
    Icosahedral plant coat protein family
  • 数据库链接：

    KEGG: vg:944390