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  4. Recombinant Type-1 restriction enzyme EcoprrI specificity protein (prrB)

Recombinant Type-1 restriction enzyme EcoprrI specificity protein (prrB)

  • 货号：
    CSB-YP322344ENL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP322344ENL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP322344ENL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP322344ENL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP322344ENL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    prrB
  • Uniprot No.：
  • 别名：
    prrB; Type-1 restriction enzyme EcoprrI specificity protein; S.EcoprrI; Type I restriction enzyme EcoprrI specificity protein; S protein
  • 种属：
    Escherichia coli
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-401
  • 氨基酸序列
    MSELSYLEKL MDGVEVEWLP LSKVFNLRNG YTPSKTKKEF WANGDIPWFR MDDIRENGRI LGSSLQKISS CAVKGGKLFP ENSILISTSA TIGEHALITV PHLANQRFTC LALKESYADC FDIKFLFYYC FSLAEWCRKN TTMSSFASVD MDGFKKFLIP RPCPDNPEKS LAIQSEIVRI LDKFSALTAE LTAELTAELS MRKKQYNYYR DQLLSFKEDE VEGKRKTLGE IMKMRAGQHI SAHNIIERKE ESYIYPCFGG NGIRGYVKEK SHDGEHLLIG RQGALCGNVQ RMKGQFYATE HAVVVSVMPG INIDWAFHML TAMNLNQYAS KSAQPGLAVG KLQELKLFVP SIERQIYIAA ILDKFDTLTN SITEVSRVKS SCARNSTNII EICYLVSRSR K
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoprrI enzyme recognizes 5'-CCAN(7)ATGC-3'.
  • 蛋白家族：
    Type-I restriction system S methylase family