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  4. Recombinant UDP-glucuronate:glycolipid 2-beta-glucuronosyltransferase (gumK)

Recombinant UDP-glucuronate:glycolipid 2-beta-glucuronosyltransferase (gumK)

  • 货号：
    CSB-YP811452XAAF
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP811452XAAF
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP811452XAAF-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP811452XAAF
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP811452XAAF
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    gumK
  • Uniprot No.：
  • 别名：
    gumKUDP-glucuronate:glycolipid 2-beta-glucuronosyltransferase; UDP-GlcA:glycolipid glucuronosyltransferase; EC 2.4.1.264; D-man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase
  • 种属：
    Xanthomonas campestris pv. campestris
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-400
  • 氨基酸序列
    MSVSPAAPAS GIRRPCYLVL SAHDFRTPRR ANIHFITDQL ALRGTTRFFS LRYSRLSRMK GDMRLPLDDT ANTVVSHNGV DCYLWRTTVH PFNTRRSWLR PVEDAMFRWY AAHPPKQLLD WMRESDVIVF ESGIAVAFIE LAKRVNPAAK LVYRASDGLS TINVASYIER EFDRVAPTLD VIALVSPAMA AEVASRDNVF HVGHGVDHNL DQLGDPSPYA EGIHAVAVGS MLFDPEFFVV ASKAFPQVTF HVIGSGMGRH PGYGDNVIVY GEMKHAQTIG YIKHARFGIA PYASEQVPVY LADSSMKLLQ YDFFGLPAVC PNAVVGPYKS RFGYTPGNAD SVIAAITQAL EAPRVRYRQC LNWSDTTDRV LDPRAYPETR LYPHPPTAAP QLSSEAALSH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the transfer of a glucuronic acid (GlcA) residue from UDP-glucuronate to mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl to form the lipid-linked tetrasaccharide GlcA-Man-Glc(2)-PP-Pol, with a glucuronic acid-beta-mannose linkage. Is involved in the biosynthesis of the exopolysaccharide xanthan, since it catalyzes the fourth glycosylation step in the assembly of the pentasaccharide-P-P-polyisoprenyl repeating unit of xanthan. Is unable to use the trisaccharide acceptor freed from the pyrophosphate lipid moiety. Does not show specificity for the lipidic portion of the acceptor. Shows diminished activity when tested with 6-O-acetyl-mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl, a putative intermediate in the synthesis of xanthan; this could indicate that acetylation of the internal mannose takes place after the formation of the GumK product.
  • 亚细胞定位：
    Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Glycosyltransferase 70 family
  • 数据库链接：

    KEGG: ag:AAN86640