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  4. Recombinant Vaccinia virus mRNA-capping enzyme regulatory subunit (VACWR117)

Recombinant Vaccinia virus mRNA-capping enzyme regulatory subunit (VACWR117)

  • 货号：
    CSB-YP356237VAI
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP356237VAI
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP356237VAI-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP356237VAI
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP356237VAI
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    VACWR117
  • Uniprot No.：
  • 别名：
    VACWR117; D12LmRNA-capping enzyme regulatory subunit; Virus termination factor small subunit; VTF small subunit; mRNA-capping enzyme 33 kDa subunit; mRNA-capping enzyme D12 subunit; mRNA-capping enzyme small subunit
  • 种属：
    Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-287
  • 氨基酸序列
    MDEIVKNIRE GTHVLLPFYE TLPELNLSLG KSPLPSLEYG ANYFLQISRV NDLNRMPTDM LKLFTHDIML PESDLDKVYE ILKINSVKYY GRSTKADAVV ADLSARNKLF KRERDAIKSN NHLTENNLYI SDYKMLTFDV FRPLFDFVNE KYCIIKLPTL FGRGVIDTMR IYCSLFKNVR LLKCVSDSWL KDSAIMVASD VCKKNLDLFM SHVKSVTKSS SWKDVNSVQF SILNNPVDTE FINKFLEFSN RVYEALYYVH SLLYSSMTSD SKSIENKHQR RLVKLLL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulatory subunit of the mRNA cap enzyme which stabilizes the catalytic subunit and enhances its methyltransferase activity through an allosteric mechanism. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the polymerase associated VP39 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure).; The heterodimeric enzyme is also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I and the Rap94 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA.
  • 基因功能参考文献：
    1. These results suggest that VTF may play a subtle role in early gene transcription elongation in addition to its known function in mRNA cap formation, early gene transcription termination and intermediate gene transcription initiation. PMID: 16460779
  • 亚细胞定位：
    Virion. Note=All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome.
  • 蛋白家族：
    Chordopoxvirinae mRNA-capping enzyme regulatory subunit family
  • 数据库链接：

    KEGG: vg:3707515