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  4. Recombinant WASH complex subunit CCDC53 homolog (ddl-1)

Recombinant WASH complex subunit CCDC53 homolog (ddl-1)

  • 货号：
    CSB-YP522324CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP522324CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP522324CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP522324CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP522324CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ddl-1
  • Uniprot No.：
  • 别名：
    ddl-1; F59E12.10WASH complex subunit homolog 3; Daf-16-dependent longevity protein 1
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-189
  • 氨基酸序列
    MNASSRTKPA IDLNKVPPID HHRTAVTFNC LIMKMTEMLN NFGNKMEDIL EKAEQSLDTA DRKLRLMESK LAGMSLEDKS TTATPSSAPE IDEIHESNPS SSQIVEETVE EKPEEHTTTV LIKDDPAYSK YFKMLKLGVL EAGVIQKMKS EGVDPSILKR GDEPSRPQAQ TSRNYESSGE STASFSDSD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Acts as a component of the DHIC (ddl-1-containing hsf-1 inhibitory complex) which modulates lifespan by sequestering the heat-shock transcription factor hsf-1 to negatively regulate its binding to DNA and its transcriptional activity.
  • 基因功能参考文献：
    1. Study shows that the proteins DDL-1 and DDL-2, previously implicated in life span extension, modulate HSF-1 activity by forming an inhibitory heterocomplex with HSF-1 and that formation of this complex is regulated by Insulin/IGF-1-like signaling. PMID: 22265419
  • 蛋白家族：
    CCDC53 family
  • 组织特异性：
    Expressed in pharynx, intestine, body wall muscles, vulva muscles, spermatheca, and several head and tail neurons.
  • 数据库链接：

    KEGG: cel:CELE_F59E12.10

    STRING: 6239.F59E12.10