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  4. Recombinant Xenopus laevis 60S ribosomal protein L5-B (rpl5-b)

Recombinant Xenopus laevis 60S ribosomal protein L5-B (rpl5-b)

  • 货号：
    CSB-YP325726XBE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP325726XBE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP325726XBE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP325726XBE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP325726XBE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rpl5-b
  • Uniprot No.：
  • 别名：
    rpl5-b; 60S ribosomal protein L5-B
  • 种属：
    Xenopus laevis (African clawed frog)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-296
  • 氨基酸序列
    GFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVKV GLTNYAAAYC TGLLLARRLL NKFGLDKVYE GQVEVTGDEY NVESVDGEPG AFTCYLDAGL TRTTTGNKVF GALKGAVDGG LSIPHSTKRF PGYDSESKEF NPEVHRKHIF AQNIAEYMRL LMEEDEDAYK KQFSQYIKNG VAADQLEDIY KKAHAGIREN PVHEKKPKKE VKKKRWNRAK LSLEQKKDRV AQKKASFLRA QEKADS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53.
  • 亚细胞定位：
    Cytoplasm. Nucleus, nucleolus.
  • 蛋白家族：
    Universal ribosomal protein uL18 family
  • 数据库链接：

    UniGene: Xl.848