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  4. Recombinant Xenopus laevis NEDD8-conjugating enzyme UBE2F (ube2f)

Recombinant Xenopus laevis NEDD8-conjugating enzyme UBE2F (ube2f)

  • 货号：
    CSB-YP746935XBE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP746935XBE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP746935XBE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP746935XBE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP746935XBE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ube2f; NEDD8-conjugating enzyme UBE2F; EC 2.3.2.32; NEDD8 carrier protein UBE2F; NEDD8-conjugating enzyme 2; Ubiquitin-conjugating enzyme E2 F
  • 种属：
    Xenopus laevis (African clawed frog)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-185
  • 氨基酸序列
    MLTLASKLKR DDGVKGSRTT STTLDSMRRI SVRDRLLVKE VAELEANLPC TCKVNFPDPN KLHYFHLTVS PDESYYQGGR FQFEIEVPDA YNMVPPKVKC LTRIWHPNIT ETGEICLSLL REHSIDGTGW APTRTLKDVV WGLNSLFTDL LNFDDPLNIE AAEHHLRDKD EYRNKVEDYI KRYAR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Accepts the ubiquitin-like protein NEDD8 from the uba3-nae1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase rbx2, but not rbx1, suggests that the rbx2-ube2f complex neddylates specific target proteins, such as cul5.
  • 蛋白家族：
    Ubiquitin-conjugating enzyme family, UBE2F subfamily
  • 数据库链接：

    KEGG: xla:432085

    UniGene: Xl.12697