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  4. Recombinant Xenopus laevis Poly (U)-specific endoribonuclease-A (endou-a)

Recombinant Xenopus laevis Poly (U)-specific endoribonuclease-A (endou-a)

  • 中文名称：
    有爪蟾蜍endou-a重组蛋白
  • 货号：
    CSB-YP809045XBE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    有爪蟾蜍endou-a重组蛋白
  • 货号：
    CSB-EP809045XBE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    有爪蟾蜍endou-a重组蛋白
  • 货号：
    CSB-EP809045XBE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    有爪蟾蜍endou-a重组蛋白
  • 货号：
    CSB-BP809045XBE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    有爪蟾蜍endou-a重组蛋白
  • 货号：
    CSB-MP809045XBE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    endou-a
  • Uniprot No.：
  • 别名：
    endou-aPoly(U)-specific endoribonuclease-A; EC 3.1.-.-; Protein endoU-A; Uridylate-specific endoribonuclease-A; XendoU-A
  • 种属：
    Xenopus laevis (African clawed frog)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-292
  • 氨基酸序列
    MASNRGQLNH ELSKLFNELW DADQNRMKSG KDYRISLQGK AGYVPAGSNQ ARDSASFPLF QFVDEEKLKS RKTFATFISL LDNYEMDTGV AEVVTPEEIA ENNNFLDAIL ETKVMKMAHD YLVRKNQAKP TRNDFKVQLY NIWFQLYSRA PGSRPDSCGF EHVFVGESKR GQEMMGLHNW VQFYLQEKRK NIDYKGYVAR QNKSRPDEDD QVLNLQFNWK EMVKPVGSSF IGVSPEFEFA LYTIVFLASQ EKMSREVVRL EEYELQIVVN RHGRYIGTAY PVLLSTNNPD LY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Poly(U)-specific endoribonuclease involved in the processing of intron-encoded box C/D snoRNAs, such as U16 and U86. Releases products that have 2',3'-cyclic phosphate termini at the 3'-end.
  • 基因功能参考文献：
    1. calcium-activated removal of RNA from membranes by XendoU promotes and refines endoplasmic reticulum remodeling and the formation of tubular endoplasmic reticulum. PMID: 25287301
    2. XendoU is the endoribonuclease involved in small nucleolar RNA biosynthesis PMID: 15755742
    3. large-scale purification and crystallization of recombinant XendoU are reported; purified monodisperse XendoU crystallized in two different space groups: trigonal P3(1)21, diffracting to low resolution, and monoclinic C2, diffracting to higher resolution PMID: 16511328
    4. Results describe the structure of XendoU that, by refined search methodologies, appears to display a unique fold. PMID: 16895992
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    ENDOU family
  • 数据库链接：

    KEGG: xla:394346

    UniGene: Xl.2569