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  4. Recombinant Xenopus laevis Serine/threonine-protein kinase PLK1 (plk1)

Recombinant Xenopus laevis Serine/threonine-protein kinase PLK1 (plk1)

  • 货号：
    CSB-YP018193XBE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018193XBE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018193XBE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018193XBE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018193XBE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    plk1; plx1; Serine/threonine-protein kinase PLK1; EC 2.7.11.21; Plx1; Polo-like kinase 1; PLK-1
  • 种属：
    Xenopus laevis (African clawed frog)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates cdc25, pkmyt1/myt1, stag2/sa2, tpx2. Plays multiple essential roles during mitosis. Phosphorylates the N-terminal domain of cdc25, which leads to cyclin b-cdc2 activation and mitotic entry. Also required for organization of bipolar spindles, and for exit from mitosis. Involved in kinetochore functions and sister chromatid cohesion by phosphorylating stag2/sa2.
  • 基因功能参考文献：
    1. Novel roles for Plk and GSK3 regulation of ADAM13 function in cranial neural crest cell migration. PMID: 25298404
    2. Plx1-mediated degradation of Bora in interphase generates oscillations in Plx1 activity and is essential for development. PMID: 24675888
    3. Coordinated interplays between Plx1 and Gwl are required for reactivation of these kinases from the G(2)/M DNA damage checkpoint and efficient checkpoint recovery. PMID: 21708943
    4. the penultimate CRS serine (Ser 101) of cyclin b1 is a Plx substrate PMID: 12612056
    5. Results suggest that polo-like kinase (Plx1) could be the missing regulator that prevents maturation-promoting factor autoamplification in stage IV oocytes. PMID: 14985258
    6. Plx1 cooperates with CaMKII to regulate cyclosome regulators, and is necessary for release of cytostatic factor metaphase arrest and sufficient when overexpressed. PMID: 16040245
    7. Data show that Plx1 couples tension signals to cellular responses through phosphorylation of the 3F3/2 epitope and targeting structural and checkpoint proteins to kinetochores. PMID: 16129782
    8. Polo-like kinase Plx1 is an essential factor for calcium ion-induced meiotic exit during cytostatic factor arrest PMID: 16407128
    9. Polo-like kinase (Plx1) phosphorylates xTRF1 in vitro and the mitotic xTRF1-chromatin association was significantly impaired when Plx1 was immunodepleted from the extracts (Plx1). PMID: 16424898
    10. Cdk1 phosphorylation of BubR1 controls spindle checkpoint arrest and plk-mediated formation of the 3F3/2 epitope. PMID: 17998400
    11. Plx1 promotes activation of Aurora A, most likely through TPX2. PMID: 19556869

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 数据库链接：

    KEGG: xla:380481

    UniGene: Xl.28141