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  4. Recombinant Xenopus laevis Sonic hedgehog protein (shh)

Recombinant Xenopus laevis Sonic hedgehog protein (shh)

  • 货号：
    CSB-YP856343XBE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP856343XBE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP856343XBE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP856343XBE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP856343XBE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    shh; Sonic hedgehog protein; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC; VHH-1; X-SHH) [Cleaved into: Sonic hedgehog protein N-product; ShhN; Shh N-terminal processed signaling domains; ShhNp)]
  • 种属：
    Xenopus laevis (African clawed frog)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-444
  • 氨基酸序列
    CGPGRG IGKRRHPKKL TPLAYKQFIP NVAEKTLGAS GRYEGKITRN SDCFKELTPN YNPDIMFKDE ESTGADRLMT QRCKDKLNAL AISVMNQWPG VKLRVTEGWD EDGHHLEESL HYEGRAVDIT TSDRDRSKYG MLGRLAVEAG FDWVYYESKA HIHCSVKAEN SVAAKSGGCF PAGARVMVEF GGTKAVKDLR PGDRVLSSDP QGNLLYSDFL MFIDQERDVK KLFYVIETSQ RKIRLTAAHL LFVAQTKVNG TRSFKSVFAS NIQPGDLIYT ADPKTMTLKA VKVEKVDLEE DTGAYAPLTA HGTVVIDQVL ASCYAVIEEH TWAHLAFAPL RFGMSLSSYI YPRDSSPPSG LQPHHQVDLQ SHHQVDLQSH HQVDLQSHHQ LEGIHWYSQL LYQIGTWLLD SNSLHPLGMA TKSS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum.; The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites. Involved in the patterning of the anterior-posterior axis of the developing limb bud. Essential for axon guidance. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO.
  • 基因功能参考文献：
    1. Hh signaling is necessary and sufficient to initiate mouth formation, and Hh activation is required in a dose-dependent fashion to determine the size of the mouth PMID: 25300580
    2. Data indicate that sonic hedgehog is expressed exclusively in the notochord but not in the spinal cord of the regenerate. PMID: 24941877
    3. Dzip1-dependent stabilization of Spop/HIB is evolutionarily conserved and essential for proper regulation of Gli/Ci proteins in the Hh pathway. PMID: 24072710
    4. These results indicate that electrical activity and second-messenger signaling mediate Shh action in embryonic spinal neurons. PMID: 21368195
    5. Connective tissue-specific expression of BMP-4 mRNA is up-regulated by sonic hedgehog. PMID: 17016847
    6. In an examination of signaling pathways in developing Xenopus lung, shh but not ihh was expressed in the very anterior part of early lung epithelium. PMID: 20682360
    7. Data propose that Shh serves as a ventral optic tract repellent that helps to define the caudal boundary for retinal axons in the diencephalon, and that this signaling is also required for initial target recognition at the optic tectum. PMID: 20931659
    8. The forebrain of Xenopus revealed a largely conserved pattern of Shh expression among tetrapods. PMID: 20540934
    9. Hedgehog regulates superficial slow muscle fibres in Xenopus and tetrapod trunk myogenesis PMID: 15201218
    10. Results suggest that 7-dehydrocholesterol reductase and Sonic Hedgehog are co-expressed during midline development in Xenopus embryos PMID: 16687448
    11. activated Hedgehog signaling in the blastema and found that target genes of Shh were inducible in the mesenchyme of limb blastema PMID: 19544583

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  • 亚细胞定位：
    Endoplasmic reticulum membrane. Golgi apparatus membrane.; [Sonic hedgehog protein N-product]: Cell membrane; Lipid-anchor.
  • 蛋白家族：
    Hedgehog family
  • 组织特异性：
    Strongly expressed in notochord and neural floor plate during embryogenesis. In tadpole, high expression is observed in pancreas/stomach, moderate expression in tail, and low expression in intestine, brain, and hind limb.
  • 数据库链接：

    KEGG: xla:398047

    UniGene: Xl.866