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  4. Recombinant Xenopus laevis Transcription factor HES-4-B (hes4-b)

Recombinant Xenopus laevis Transcription factor HES-4-B (hes4-b)

  • 中文名称：
    有爪蟾蜍hes4-b重组蛋白
  • 货号：
    CSB-YP835464XBE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    有爪蟾蜍hes4-b重组蛋白
  • 货号：
    CSB-EP835464XBE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    有爪蟾蜍hes4-b重组蛋白
  • 货号：
    CSB-EP835464XBE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    有爪蟾蜍hes4-b重组蛋白
  • 货号：
    CSB-BP835464XBE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    有爪蟾蜍hes4-b重组蛋白
  • 货号：
    CSB-MP835464XBE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    hes4-b
  • Uniprot No.：
  • 别名：
    hes4-b; hairy2; hairy2b; Transcription factor HES-4-B; Hairy and enhancer of split 4-B; Protein hairy-2; Xhairy2; Protein hairy-2a; Xhairy2b
  • 种属：
    Xenopus laevis (African clawed frog)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-277
  • 氨基酸序列
    MPADSMEKPT ASPIAGAPAN SAQTPDKPKS ASEHRKSSKP IMEKRRRARI NESLGQLKTL ILDALKKDSS RHSKLEKADI LEMTVKHLRN LQRVQMTAAL TADPSVLGKY RAGFNECMNE VTRFLSTCEG VNTEVRTRLL GHLSSCLGQI VAMNYQQPPS SQQPVHVQLP SSTPVPMPCK VNPAEAISPK VFQGGFQLVP ATDGQFAFLI PNPAYTSSPG PVIPLYANAT SPGGPPSQSP VQGLTSFAHK MPHMAQAVSP LGGSTRADSA ESVWRPW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor. Binds DNA on N-box motifs: 5'-CACNAG-3'. Promotes floor plate development and prechordal plate development. Required for lens development as early as the stage of lens field formation, partly through regulation of gene expression of the cell cycle inhibitor cdknx/p27(xic1). Required for formation of the neural crest downstream of multiple signaling pathways, and acts at the neural plate border via both DNA-binding dependent and independent mechanisms; acts in a DNA-binding dependent manner to repress pro-apoptotic and neural crest differentiation genes, including id3, delta1, and cdknx/p27(xic1), and thus promote the cell survival of neural plate border cells and maintain them in an undifferentiated state. Represses transcription of id3, at least in part through the repression of bmp4. On the other hand, acts in a DNA-independent manner separate from the transcriptional repressor function, to stimulate cell proliferation and promote neural crest formation. Via this DNA-independent route, acts in neurulae upstream of stat3 to transiently up-regulate the notch ligand dll1/delta1, which in turn up-regulates id3 expression. Then interacts directly with id3, which blocks the transcriptional repressor function of hes4-B/hairy2b to allow the progression of neural crest progenitors through specification and differentiation. Also acts via repressor-dependent and repressor-independent mechanisms in early gastrulae to establish the prospective anterior prechordal mesoderm identity in the Spemann organizer; induces specific genes independently from direct transcriptional regulation, and represses the genes specific for neighboring tissues through direct transcriptional repression. Modulates lateral inhibition during notch signaling and regulates the cell context dependent effects of notch (which can have inhibitory, permissive or enhancing roles in muscle or neural differentiation). Inhibits myogenesis.
  • 基因功能参考文献：
    1. hairy2b shows extensive overlap of expression with HRT1 in floor plate precursors and may be a biologically relevant HRT1 partner. PMID: 15531363
    2. Establishes the identity of the anterior prechordal mesoderm within Spemann's organizer by inhibiting the formation of neighboring tissues. PMID: 16059909
  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Dynamically expressed in the borders of several tissue territories. Expressed in the pre-placodal ectoderm (PPE) from gastrula stage. During gastrulation, expressed in the deep layer of the dorsal lip, the Spemann organizer and three distinct regions in t
  • 数据库链接：

    KEGG: xla:398258

    UniGene: Xl.86822