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  4. Recombinant Xenopus laevis Zinc finger protein ZIC 1 (zic1)

Recombinant Xenopus laevis Zinc finger protein ZIC 1 (zic1)

  • 货号：
    CSB-YP026484XBE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP026484XBE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP026484XBE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP026484XBE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP026484XBE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    zic1; oplZinc finger protein ZIC 1; XZic1; XlZic1; ODD-paired-like; Xopl; ZIC-related protein 1; ZIC-r1; Zinc finger protein of the cerebellum 1
  • 种属：
    Xenopus laevis (African clawed frog)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-443
  • 氨基酸序列
    MLLDAGAQYP AIGVTTFGSS RHHSAGDVTD REVALGINPF ADGMGAFKLN PSSHDLASGQ TAFTSQAPGY AAAALGHHHH PGHVSSYSSA AFNSTRDFLF RNRGFGEAAS AQHSLFASAA GGFPGPHGPH ADTTGHLIFP GLHEQAASHA SPNVVNGQMR LGFSGDMYGR PDQYGQVTSP RSEHYASSQL HGYGPMNMNM AAHHGAGAFF RYMRQPIKQE LICKWIEPEQ LANPKKSCNK TFSTMHELVT HVTVEHVGGP EQSNHICVWE ECPREGKPFK AKYKLINHIR VHTGEKPFPC PFPGCGKVFA RSENLKIHKR THTGEKPFKC EFEGCDRRFA NSSDRKKHMH VHTSDKPYLC KMCDKSYTHP SSLRKHMKVH EASSQGSQPS PAASSGYESS TPPTIVSPSA ENQSTSSLSP SSSAVHHTSN HSTLSSNFNE WYV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator that induces expression of multiple genes including pax3, en2, snai2/slug, feb and a subset of wnt genes. Has multiple key roles in the regulation of neural induction and neurogenesis: acts as a neural competence factor, sensitizing the presumptive neuroectoderm to respond to subsequent neuralizing signals. Promotes both preplacodal cell fates and neural crest cell fates, two of the cell populations that arise from the neural plate border. Cooperates with pax3 in concert with wnt signaling to determine neural crest fate. Synergizes with the bmp-inhibitor noggin/nog and acts through the wnt pathway to induce expression of en2. May bind to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.
  • 基因功能参考文献：
    1. Zic1, expressed at the anterior neural plate, is necessary and sufficient to promote placode fate. PMID: 26101153
    2. Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers. PMID: 24360906
    3. Pax3 and Zic1 drive induction and differentiation of multipotent, migratory, and functional neural crest in Xenopus embryos. PMID: 23509273
    4. Co-activation of Pax3 and Zic1, in concert with Wnt, plays a decisive role for early neural crest determination in the correct place of the Xenopus ectoderm. PMID: 15843410
    5. These data suggest that interruption of BMP signaling facilitates neural determination via a complex mechanism, involving multiple regulatory factors that cooperate to control zic1 expression PMID: 16307736
    6. Zic1 directly upregulated the Xfeb gene during early neural development. PMID: 16871635
    7. Zic1 is an activator of Wnt signaling. PMID: 16892174
    8. Data show that Pax3 and Zic1 are necessary and sufficient to promote hatching gland and preplacodal fates, respectively, and that their combined activity is essential to specify the neural crest. PMID: 17409353
    9. aqp-3b was upregulated by Zic1 PMID: 19384961

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    GLI C2H2-type zinc-finger protein family
  • 组织特异性：
    During early gastrula stages, widely expressed in the dorsal ectoderm. At mid-gastrula, expressed throughout the presumptive neural plate and at late gastrula, expression gradually diminishes in the dorsal midline and increases in the anterior folds. By e
  • 数据库链接：

    KEGG: xla:399124

    UniGene: Xl.1796