Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Zea mays Polyamine oxidase (PAO)

Recombinant Zea mays Polyamine oxidase (PAO)

  • 货号：
    CSB-YP524626ZAX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP524626ZAX
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP524626ZAX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP524626ZAX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP524626ZAX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PAO
  • Uniprot No.：
  • 别名：
    MPAO1; MPAO; PAO; Polyamine oxidase 1; EC 1.5.3.14; EC 1.5.3.15
  • 种属：
    Zea mays (Maize)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    29-500
  • 氨基酸序列
    AT VGPRVIVVGA GMSGISAAKR LSEAGITDLL ILEATDHIGG RMHKTNFAGI NVELGANWVE GVNGGKMNPI WPIVNSTLKL RNFRSDFDYL AQNVYKEDGG VYDEDYVQKR IELADSVEEM GEKLSATLHA SGRDDMSILA MQRLNEHQPN GPATPVDMVV DYYKFDYEFA EPPRVTSLQN TVPLATFSDF GDDVYFVADQ RGYEAVVYYL AGQYLKTDDK SGKIVDPRLQ LNKVVREIKY SPGGVTVKTE DNSVYSADYV MVSASLGVLQ SDLIQFKPKL PTWKVRAIYQ FDMAVYTKIF LKFPRKFWPE GKGREFFLYA SSRRGYYGVW QEFEKQYPDA NVLLVTVTDE ESRRIEQQSD EQTKAEIMQV LRKMFPGKDV PDATDILVPR WWSDRFYKGT FSNWPVGVNR YEYDQLRAPV GRVYFTGEHT SEHYNGYVHG AYLSGIDSAE ILINCAQKKM CKYHVQGKYD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Flavoenzyme involved in polyamine back-conversion (Ref.4, PubMed:16331971). Catalyzes the oxidation of the secondary amino group of polyamines, such as spermine, spermidine and their acetyl derivatives (Ref.4, PubMed:16331971). Plays an important role in the regulation of polyamine intracellular concentration (Probable).
  • 基因功能参考文献：
    1. Underexpression of Zea mays polyamine oxidase (ZmPAO) correlates with increased thermotolerance of the photosynthetic machinery and improved biomass accumulation, accompanied by enhanced levels of the enzymatic and non-enzymatic antioxidants, whereas ZmPAO overexpressors exhibit significant impairment of thermotolerance. PMID: 28886452
    2. deregulation of the apoplastic PAO can be further examined as a potential approach to breed plants with enhanced/reduced tolerance to abiotic stress with minimal associated trade-offs. PMID: 28135604
    3. data suggest that H2O2 produced through PA catabolism by ZmPAO1 plays an important role in tumor development during the maize-U. maydis interaction. PMID: 26926794
    4. Rsults indicate that the HOH309-K300 couple plays a major role in multiple steps of ZmPAO catalytic mechanism, such as correct substrate binding geometry as well as FAD reduction and reoxidation kinetics. PMID: 21205212
    5. Data indicate the importance of lysine300 and probable importance of water309 to the mechanism of flavin reduction in maize polyamine oxidase. PMID: 16331971
    6. This study evaluated the effect of wounding on polyamine oxidase expression in the outer tissues of the maize mesocotyl and provide evidence that its activity, protein, and mRNA levels increased in response to wounding as well as jasmonic acid treatment. PMID: 17993545
    7. MPAO contributes to abscisic acid-induced cytosolic antioxidant defense through hydrogen peroxide, a Spermidine catabolic product. PMID: 19261065
    8. PAO contributes to sustain maize leaf elongation under saline stress. PMID: 19717530

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted, extracellular space, apoplast. Secreted, cell wall.
  • 蛋白家族：
    Flavin monoamine oxidase family
  • 数据库链接：

    KEGG: zma:541983

    STRING: 4577.GRMZM2G034152_P01

    UniGene: Zm.300