Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 标签内参抗体
  4. ACTB Monoclonal Antibody

ACTB Monoclonal Antibody

  • 货号：
    CSB-MA000091M2m
  • 规格：
    ￥400
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue, Rat heart tissue, Rat skeletal muscle tissue, Rabbit lung tissue, Rabbit liver tissue
      All lanes: ACTB antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: 293T whole cell lysate, Zebrafish tissue
      All lanes: ACTB antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse skeletal muscle tissue
      All lanes: ACTB antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: Rat heart tissue, Rat liver tissue, Rat kidney tissue, U87 whole cell lysate Rat skeletal muscle tissue
      All lanes: ACTB antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue, Rabbit lung tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue, A549 whole cell lysate, MCF-7 whole cell lysate
      All lanes: ACTB antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: Hela whole cell lysate at 40μg, 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg
      All lanes: ACTB antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: 20μg Hela whole cell lysate
      ACTB antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
  • 其他：

产品详情

  • 产品描述：

    The ACTB monoclonal antibody is created using the synthesized peptide derived from human Beta-Actin (1-50aa) to immunize the mouse, with hybridoma cells being generated by fusing mouse B cells (isolated from the immunized mouse) with myeloma cells. The hybridoma cells that produce the ACTB antibody are then selected and cultured, and the ACTB monoclonal antibody is purified from the mouse ascites using protein G affinity chromatography. This unconjugated IgG1 antibody is highly specific to the ACTB proteins from four species including human, mouse, rat, and rabbit. It can be used in ELISA and WB assays.

    ACTB, also known as beta-actin, is a protein that plays a structural role in the cytoskeleton of eukaryotic cells. It is involved in maintaining cell shape, adhesion, and motility, and is also important for intracellular transport and cytokinesis. Additionally, ACTB is involved in gene transcription and chromatin remodeling and has been shown to interact with a variety of proteins involved in signaling pathways and cellular processes.

  • 基因名：
  • 别名：
    ACTB;ACTB;ACTB;PS1TP5BP1;beta-ac6n;β-ac6n
  • 宿主：
    Mouse
  • 反应种属：
    Human, Mouse, Rat, Rabbit
  • 免疫原：
    A synthesized peptide derived from human Beta-Actin (1-50AA)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG1
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    6D8D8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:10000-1:1280000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价